Xu Jianfeng, Wang Yusheng, Yang Xiumei, Zhang Peng, Chen Lijun
Department of Ophthalmology, Institute of Ophthalmology of Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Yan Ke Xue Bao. 2006 Sep;22(3):166-74, 183.
Choroidal neovascularization (CNV) plays an important role in pathogenesis of age-related macular degeneration (AMD), ocular histoplasmosis syndrome (OHS) and so on. However, mechanisms of CNV formation are not fully understood. The aim of this study is to investigate the correlation between expressions of CD105 and vascular endothelial growth factor (VEGF) in experimental laser-induced CNV in rats.
CNV model was established by 532 nm laser photocoagulation in Brown-Norway rats. The expression of CD105 and VEGF in CNV was observed by immunohistochemistry at 3, 7, 14, 21, 28 and 56 days after laser photocoagulation. The image analysis was performed with the professional software of Image-Pro Plus.
Fluorescein angiography showed fluorescein leakage in CNV from days 7 to 56 after photocoagulation. VEGF expression was mainly observed in vascular endothelial cells, ganglion cells, inner nuclear layers and retinal pigment epithelial cells in normal retina and vascular endothelial cells in normal choroid of the rats. On day 3 after photocoagulation, VEGF began to express in laser-induced lesions. VEGF was strongly expressed in CNV after 7 days (P < 0.05) and decreased after 14 days (P > 0.05). CD105 was initially presented in CNV at 7 days and obviously expressed at 14 days after photocoagulation (P < 0.05). Four weeks later, when angiogenesis tended toward inactive status, expression of CD105 was markedly decreased (P>0.05). There was notablely direct correlation between CD105-positive-microvessel density and positively semiquantitative scoring of VEGF in the CNV (r = 0.989, P < 0.01).
There is direct correlation between the expression of CD105 and VEGF in the laser-induced CNV in rat. It suggests that CD105 and VEGF might participate in the new blood vessel formation and promote the growth of CNV.
脉络膜新生血管（CNV）在年龄相关性黄斑变性（AMD）、眼组织胞浆菌病综合征（OHS）等疾病的发病机制中起重要作用。然而，CNV形成的机制尚未完全阐明。本研究旨在探讨大鼠实验性激光诱导CNV中CD105与血管内皮生长因子（VEGF）表达之间的相关性。
采用532nm激光光凝法在Brown-Norway大鼠中建立CNV模型。于激光光凝后3、7、14、21、28和56天，通过免疫组织化学观察CNV中CD105和VEGF的表达情况。使用Image-Pro Plus专业软件进行图像分析。
荧光血管造影显示光凝后7至56天CNV中有荧光素渗漏。VEGF表达主要见于正常视网膜的血管内皮细胞、神经节细胞、内核层和视网膜色素上皮细胞以及大鼠正常脉络膜的血管内皮细胞。光凝后第3天，VEGF开始在激光诱导的损伤部位表达。7天后VEGF在CNV中强烈表达（P<0.05），14天后表达下降（P>0.05）。CD105在光凝后7天开始在CNV中出现，14天时明显表达（P<0.05）。四周后，当血管生成趋于静止状态时，CD105表达明显下降（P>0.05）。CNV中CD105阳性微血管密度与VEGF阳性半定量评分之间存在显著正相关（r = 0.989，P<0.01）。
大鼠激光诱导CNV中CD105与VEGF的表达之间存在直接相关性。这表明CD105和VEGF可能参与新生血管形成并促进CNV的生长。
