通过逆流色谱法从菠菜中分离叶绿素a和叶绿素b。
Isolation of chlorophylls a and b from spinach by counter-current chromatography.
作者信息
Jubert Carole, Bailey George
机构信息
Linus Pauling Institute, Oregon State University, 435 Weniger Hall, Corvallis, OR 97331, USA.
出版信息
J Chromatogr A. 2007 Jan 26;1140(1-2):95-100. doi: 10.1016/j.chroma.2006.11.063. Epub 2006 Dec 11.
A method for the isolation of chlorophylls from spinach by counter-current chromatography was developed. An initial extraction protocol was devised to avoid the notorious sensitivity of chlorophylls to degradation by light, heat, oxygen, acids and bases. Further purification and separation of chlorophylls a and b were achieved using counter-current chromatography. Chlorophyll structures and purities were established by HPLC, fast atom bombardment mass spectrometry and nuclear magnetic resonance. Purity was estimated to be >95% (100% by HPLC). Typical yields from 30g of freeze-dried spinach were 300mg of chlorophyll a and 100mg of chlorophyll b.
开发了一种通过逆流色谱法从菠菜中分离叶绿素的方法。设计了初始提取方案，以避免叶绿素对光、热、氧、酸和碱降解的敏感性。使用逆流色谱法进一步纯化和分离叶绿素a和叶绿素b。通过高效液相色谱法、快原子轰击质谱法和核磁共振确定叶绿素的结构和纯度。估计纯度>95%（高效液相色谱法测定为100%）。30克冻干菠菜的典型产量为300毫克叶绿素a和100毫克叶绿素b。
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