Chang J M, Johnson G R
Cancer Research Unit, Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Exp Hematol. 1991 Aug;19(7):602-7.
C57BL/6 mice were established to constitutively produce multi-colony-stimulating factor (multi-CSF), granulocyte-macrophage colony-stimulating factor, or granulocyte colony-stimulating factor by transplantation with post-5-fluorouracil-treated syngeneic marrow cells infected with retroviral vectors bearing the corresponding growth factor complementary DNAs. At 2-4 weeks after transplantation, these mice had a 2-7-fold increase in spleen colony-forming unit (CFU-S) numbers when compared to control animals transplanted with MPZipNeo-infected marrow cells. Most of the CFU-S in the former animals were located in the spleen, whereas those of control mice were found in the marrow. The increase in total CFU-S content in recipients of CSF-infected mice was not due to an increase in self-renewal ability but rather to the recruitment of more primitive cells, as there were no differences in CFU-S content of spleen colonies generated from marrow cells of either group. Neither were there any differences in the cellular composition of these spleen colonies or in the size or distribution of cell types in secondary spleen colonies generated from these spleen colonies, suggesting the inability of any of the three CSFs to alter the differentiation pattern of CFU-S.
通过移植感染携带相应生长因子互补DNA的逆转录病毒载体的经5-氟尿嘧啶处理的同基因骨髓细胞，建立了组成性产生多集落刺激因子（multi-CSF）、粒细胞-巨噬细胞集落刺激因子或粒细胞集落刺激因子的C57BL/6小鼠。移植后2-4周，与移植感染MPZipNeo的骨髓细胞的对照动物相比，这些小鼠的脾集落形成单位（CFU-S）数量增加了2-7倍。前一组动物中的大多数CFU-S位于脾脏中，而对照小鼠的CFU-S则存在于骨髓中。CSF感染小鼠受体中总CFU-S含量的增加不是由于自我更新能力的提高，而是由于招募了更多原始细胞，因为两组骨髓细胞产生的脾集落的CFU-S含量没有差异。这些脾集落的细胞组成以及由这些脾集落产生的二级脾集落中的细胞类型大小或分布也没有任何差异，这表明三种CSF中的任何一种都无法改变CFU-S的分化模式。
