正常结膜组织和结膜乳头状瘤中的人乳头瘤病毒：大量病例中的类型及频率
Human papillomavirus in normal conjunctival tissue and in conjunctival papilloma: types and frequencies in a large series.
作者信息
Sjö Nicolai Christian, von Buchwald Christian, Cassonnet Patricia, Norrild Bodil, Prause Jan Ulrik, Vinding Troels, Heegaard Steffen
机构信息
Eye Pathology Institute, University of Copenhagen, Frederik V's Vej 11, DK-2100 Copenhagen Ø, Denmark.
出版信息
Br J Ophthalmol. 2007 Aug;91(8):1014-5. doi: 10.1136/bjo.2006.108811. Epub 2006 Dec 13.
AIM
To examine conjunctival papilloma and normal conjunctival tissue for the presence of human papillomavirus (HPV).
METHODS
Archival paraffin wax-embedded tissue from 165 conjunctival papillomas and from 20 histological normal conjunctival biopsy specimens was analysed for the presence of HPV by PCR. Specimens considered HPV positive using consensus primers, but with a negative or uncertain PCR result using type-specific HPV probes, were analysed with DNA sequencing.
RESULTS
HPV was present in 86 of 106 (81%) beta-globin-positive papillomas. HPV type 6 was positive in 80 cases, HPV type 11 was identified in 5 cases and HPV type 45 was present in a single papilloma. All the 20 normal conjunctival biopsy specimens were beta-globin positive and HPV negative.
CONCLUSION
There is a strong association between HPV and conjunctival papilloma. The study presents the largest material of conjunctival papilloma investigated for HPV and the first investigation of HPV in normal conjunctival tissue. HPV types 6 and 11 are the most common HPV types in conjunctival papilloma. This also is the first report of HPV type 45 in conjunctival papilloma.
目的
检测结膜乳头瘤及正常结膜组织中人乳头瘤病毒（HPV）的存在情况。
方法
采用聚合酶链反应（PCR）分析165例结膜乳头瘤存档石蜡包埋组织及20例组织学正常的结膜活检标本中HPV的存在情况。对于使用通用引物检测HPV呈阳性，但使用型特异性HPV探针检测PCR结果为阴性或不确定的标本，进行DNA测序分析。
结果
106例β-珠蛋白阳性的乳头瘤中，86例（81%）检测到HPV。80例HPV 6型呈阳性，5例检测到HPV 11型，1例乳头瘤中存在HPV 45型。所有20例正常结膜活检标本β-珠蛋白呈阳性，HPV呈阴性。
结论
HPV与结膜乳头瘤之间存在密切关联。本研究提供了检测HPV的最大规模结膜乳头瘤样本，并首次对正常结膜组织中的HPV进行了研究。HPV 6型和11型是结膜乳头瘤中最常见的HPV类型。这也是结膜乳头瘤中HPV 45型的首次报道。
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