Carvalho Beatriz, Buffart Tineke E, Reis Rui M, Mons Thomas, Moutinho Cátia, Silva Paula, van Grieken Nicole C T, Grabsch Heike, van de Velde Cornelis J H, Ylstra Bauke, Meijer Gerrit A, Carneiro Fátima
Department of Pathology, VUMC - VU University Medical Centre, Amsterdam, The Netherlands.
Cell Oncol. 2006;28(5-6):283-94. doi: 10.1155/2006/650620.
Gastric cancer is one of the most frequent malignancies in the world. Nonetheless, the knowledge of the molecular events involved in the development of gastric carcinoma is far from complete. One of the hallmarks of gastric cancer is chromosomal instability resulting in abnormal DNA copy number changes throughout the genome. Mixed gastric carcinomas constitute a rare histological entity, containing the two main histological phenotypes (diffuse and intestinal). Very little is known about the underlying mechanisms of phenotypic divergence in these mixed tumours. To the best of our knowledge only E-Cadherin mutations were implicated so far in the divergence of these tumours and nothing is known about the involvement of chromosome copy number changes in the two divergent histological components. In this study, we compared the DNA copy number changes, in the two different components (diffuse and intestinal) of mixed gastric carcinomas by microarray - comparative genomic hybridisation (array CGH). The analysis of 12 mixed gastric carcinomas showed no significant differences in array CGH profiles between the diffuse and intestinal components of mixed carcinomas. This supports the idea that the phenotypic divergence within mixed gastric carcinomas is not caused by DNA chromosomal aberrations.
胃癌是全球最常见的恶性肿瘤之一。然而，对于胃癌发生过程中所涉及的分子事件的了解还远远不够完善。胃癌的一个特征是染色体不稳定，导致整个基因组中DNA拷贝数发生异常变化。混合型胃癌是一种罕见的组织学实体，包含两种主要的组织学表型（弥漫型和肠型）。对于这些混合性肿瘤中表型差异的潜在机制知之甚少。据我们所知，到目前为止，只有E-钙黏蛋白突变与这些肿瘤的差异有关，而关于染色体拷贝数变化在两种不同组织学成分中的作用尚无定论。在本研究中，我们通过微阵列比较基因组杂交（阵列CGH）比较了混合型胃癌两种不同成分（弥漫型和肠型）中的DNA拷贝数变化。对12例混合型胃癌的分析显示，混合型癌的弥漫型和肠型成分在阵列CGH图谱上没有显著差异。这支持了混合型胃癌内表型差异并非由DNA染色体畸变引起的观点。
