促胰液素和蛙皮素对培养的胰腺腺泡细胞中酶的影响。

Effects of secretin and caerulein on enzymes of cultured pancreatic acinar cells.

作者信息

Hirschi K K, Kenny S, Justice J D, Brannon P M

机构信息

Department of Nutrition and Food Science and Nutritional Sciences Program, University of Arizona, Tucson 85721.

出版信息

In Vitro Cell Dev Biol. 1991 Aug;27A(8):660-4. doi: 10.1007/BF02631110.

DOI:10.1007/BF02631110

PMID:1717428

Abstract

We examined the effects of secretin (0 to 200 nM) and caerulein (0 to 100 nM) on rat pancreatic acinar cells cultured 0 to 48 h in serum-free medium. The effects of 100 nM secretin with 1 nM caerulein were also studied because secretin may potentiate the effects of caerulein. Cellular and media (secreted) lipase and amylase were analyzed as were cellular DNA and protein content. Cellular lipase and amylase activities significantly decreased (P less than 0.0001) over time in all treatment groups, whereas media amylase and lipase significantly increased (P less than 0.0001). Neither secretin nor caerulein affected cellular lipase or media amylase. However, secretin significantly increased (P less than 0.04) and caerulein tended to increase (P less than 0.08) media lipase in a dose-dependent manner. At 12 h, 10 nM secretin maximally increased media lipase (58%) suggesting that cultured acinar cells remain responsive to secretin in vitro. Caerulein, at all concentrations, significantly decreased (P less than 0.001) cellular amylase but exhibited a dose-dependent effect only at 24 h when 100 nM caerulein maximally decreased cellular amylase (34%). Secretin (100 nM) did not alter these effects of caerulein. These results support the proposed role of caerulein in the regulation of amylase but not a direct role of secretin in the regulation of lipase.

摘要

我们研究了促胰液素（0至200 nM）和蛙皮素（0至100 nM）对在无血清培养基中培养0至48小时的大鼠胰腺腺泡细胞的影响。还研究了100 nM促胰液素与1 nM蛙皮素的联合作用，因为促胰液素可能增强蛙皮素的作用。分析了细胞和培养基（分泌的）脂肪酶和淀粉酶，以及细胞DNA和蛋白质含量。在所有处理组中，细胞脂肪酶和淀粉酶活性随时间显著降低（P<0.0001），而培养基淀粉酶和脂肪酶显著增加（P<0.0001）。促胰液素和蛙皮素均不影响细胞脂肪酶或培养基淀粉酶。然而，促胰液素以剂量依赖性方式显著增加（P<0.04），蛙皮素倾向于增加（P<0.08）培养基脂肪酶。在12小时时，10 nM促胰液素使培养基脂肪酶最大增加（58%），表明培养的腺泡细胞在体外对促胰液素仍有反应。在所有浓度下，蛙皮素均显著降低（P<0.001）细胞淀粉酶，但仅在24小时时表现出剂量依赖性效应，此时100 nM蛙皮素使细胞淀粉酶最大降低（34%）。促胰液素（100 nM）并未改变蛙皮素的这些作用。这些结果支持了蛙皮素在淀粉酶调节中的作用，但不支持促胰液素在脂肪酶调节中的直接作用。