通过异源序列重组可形成用于Qβ复制酶的高效模板。

Efficient templates for Q beta replicase are formed by recombination from heterologous sequences.

作者信息

Munishkin A V, Voronin L A, Ugarov V I, Bondareva L A, Chetverina H V, Chetverin A B

机构信息

Institute of Protein Research, Academy of Sciences, Moscow, U.S.S.R.

出版信息

J Mol Biol. 1991 Sep 20;221(2):463-72. doi: 10.1016/0022-2836(91)80067-5.

DOI:10.1016/0022-2836(91)80067-5

PMID:1717699

Abstract

A very efficient replicase template has been isolated from the products of spontaneous RNA synthesis in an in vitro Q beta replicase reaction that was incubated in the absence of added RNA. This template was named RQ135 RNA because it is 135 nucleotides in length. Its sequence consists entirely of segments that are homologous to ribosomal 23 S RNA and the phage lambda origin of replication. The sequence segments are unrelated to the sequence of Q beta bacteriophage genomic RNA. Nonetheless, this natural recombinant is replicated in vitro at a rate equal to the most efficient of the known Q beta RNA variants. Apparently, the structural properties that ensure recognition of an RNA template by Q beta replicase are not confined to viral RNA, but can appear as a result of recombination among other RNAs that usually occur in cells.

摘要

在不添加RNA的情况下进行体外Qβ复制酶反应时，从自发RNA合成产物中分离出了一种非常高效的复制酶模板。该模板被命名为RQ135 RNA，因为它长度为135个核苷酸。其序列完全由与核糖体23S RNA和噬菌体λ复制起点同源的片段组成。这些序列片段与Qβ噬菌体基因组RNA的序列无关。尽管如此，这种天然重组体在体外的复制速度与已知最有效的QβRNA变体相同。显然，确保Qβ复制酶识别RNA模板的结构特性并不局限于病毒RNA，而是可以通过细胞中通常发生的其他RNA之间的重组产生。