TaqMan、MGB、分子信标和基于SYBR Green的检测方法在检测和定量抗草甘膦转基因大豆中的性能相当。

Equal performance of TaqMan, MGB, molecular beacon, and SYBR green-based detection assays in detection and quantification of roundup ready soybean.

作者信息

Andersen Charlotte Bøydler, Holst-Jensen Arne, Berdal Knut Gunnar, Thorstensen Tage, Tengs Torstein

机构信息

Section of Food and Feed Microbiology, National Veterinary Institute, Ullevaalsveien 68, 0454 Oslo, Norway.

出版信息

J Agric Food Chem. 2006 Dec 27;54(26):9658-63. doi: 10.1021/jf061987c.

DOI:10.1021/jf061987c

PMID:17177484

Abstract

We have tested and compared the performance of 12 different assays representing four different real-time polymerase chain reaction (PCR) chemistries in the context of genetically modified organism detection. Several different molecular beacon, SYBR Green, TaqMan, and MGB assays were designed for the event specific detection and quantification of the 3' integration junction of GTS 40-3-2 (Roundup Ready) soybean. Sensitivity as well as robustness in the presence of background DNA were tested. None of the PCR-based approaches appeared to be significantly better than any of the other, but the molecular beacon assays had the lowest efficiency and also seemed more sensitive to changes in experimental setup.

摘要

我们已经测试并比较了12种不同检测方法的性能，这些方法代表了四种不同的实时聚合酶链反应（PCR）化学方法，用于转基因生物检测。针对GTS 40-3-2（抗农达）大豆3'整合接头的事件特异性检测和定量，设计了几种不同的分子信标、SYBR Green、TaqMan和MGB检测方法。测试了背景DNA存在时的灵敏度和稳健性。基于PCR的方法似乎都不比其他方法显著优越，但分子信标检测方法效率最低，而且似乎对实验设置的变化更敏感。