土壤样本中抗草甘膦转基因玉米和抗草甘膦转基因大豆转基因的实时聚合酶链反应定量分析。
Real-time polymerase chain reaction quantification of the transgenes for roundup ready corn and roundup ready soybean in soil samples.
作者信息
Lerat S, England L S, Vincent M L, Pauls K P, Swanton C J, Klironomos J N, Trevors J T
机构信息
Department of Environmental Biology, of Plant Agriculture, and of Botany, University of Guelph, Guelph, Ontario, Canada.
出版信息
J Agric Food Chem. 2005 Mar 9;53(5):1337-42. doi: 10.1021/jf048830+.
A method for quantification of recombinant DNA for Roundup Ready (RR) corn and RR soybean in soil samples is described. Soil DNA from experimental field samples was extracted using a soil DNA extraction kit with a modified protocol. For the detection and quantification of recombinant DNA of RR corn and RR soybean, a molecular beacon and two pairs of specific primers were designed to differentially target recombinant DNA in these two genetically modified crops. Soil DNA extracts were spiked with RR corn or RR soybean DNA, and recombinant DNA was quantified using real-time PCR with a molecular beacon. As few as one copy of RR corn genome or one copy of RR soybean genome was detected in the soil DNA extract.
本文描述了一种用于定量土壤样品中抗农达（RR）玉米和RR大豆重组DNA的方法。使用改良方案的土壤DNA提取试剂盒从试验田样品中提取土壤DNA。为了检测和定量RR玉米和RR大豆的重组DNA，设计了一个分子信标和两对特异性引物，以分别靶向这两种转基因作物中的重组DNA。向土壤DNA提取物中加入RR玉米或RR大豆DNA，然后使用带有分子信标的实时PCR对重组DNA进行定量。在土壤DNA提取物中检测到低至一份RR玉米基因组或一份RR大豆基因组。
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