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[采用免疫酶法检测冻干毛细血管血样中的结核抗体]

[Determination of tuberculosis antibodies using an immunoenzyme assay in lyophilized samples of capillary blood].

作者信息

Vishnevskaia E B

出版信息

Lab Delo. 1991(6):38-40.

PMID:1717752
Abstract

The aim of this work was to find the technique of enzyme immunoassay (EIA) of capillary blood lyophilized on a filter paper disk. The author has selected a buffer on the basis of egg yolk aqueous extract, that permits blocking of nonspecific binding in EIA but not preventing the specific antigen-antibody interactions. The results of analyses of capillary blood lyophilized drops and of blood serum samples, carried out in 7 tuberculous patients and 6 donors, coincided. The results were tested in 57 blood samples.

摘要

这项工作的目的是找到一种用于检测滤纸片上冻干的毛细血管血的酶免疫分析(EIA)技术。作者基于蛋黄水提取物选择了一种缓冲液,该缓冲液可在酶免疫分析中封闭非特异性结合,但不阻止特异性抗原 - 抗体相互作用。对7名结核病患者和6名献血者的毛细血管血冻干滴及血清样本进行的分析结果相符。这些结果在57份血样中得到了验证。

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