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源自感染鼠γ-疱疹病毒78的小鼠的肿瘤细胞系的建立与鉴定

Establishment and characterization of a tumor cell line derived from a mouse infected with murine gammaherpesvirus 78.

作者信息

Mistríková J, Blaskovicová J, Pappová M, Hricová M

机构信息

Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 84215 Bratislava, Slovak Republic.

出版信息

Acta Virol. 2006;50(4):223-7.

Abstract

A tumor cell line, NB-78, was derived from a lymphoma from a BALB/c mouse infected with Murine gammaherpesvirus 78 (MHV-78). Cultures of the cells of this line underwent till now more than 100 passages, displaying an epitheloid transformed morphology and a diploid complement of 40 chromosomes. Viral antigen was detected in 12% of cells by immunofluorescence (IF) test. Reactivation of latent MHV-78 was proved by detecting infectious virus in culture medium only at passages 4345. The presence of viral M1, M2, M3, and M4 gene sequences in the genome of the cells was demonstrated by PCR. NB-78 is the first continuous cell line, which originates from a tumor of a MHV-78-infected host, harbors viral genome or at least its several genes, and produces infectious virus only rarely upon reactivation. It can be assumed that this cell line is primarily associated with MHV-78 and will serve as an invaluable tool for studying the MHV-78 latency.

摘要

肿瘤细胞系NB - 78源自一只感染了鼠γ - 疱疹病毒78(MHV - 78)的BALB/c小鼠的淋巴瘤。该细胞系的细胞培养至今已传代100多次,呈现上皮样转化形态,具有40条染色体的二倍体核型。通过免疫荧光(IF)试验在12%的细胞中检测到病毒抗原。仅在第43 - 45代时,通过在培养基中检测到感染性病毒,证实了潜伏的MHV - 78被激活。通过PCR证明细胞基因组中存在病毒M1、M2、M3和M4基因序列。NB - 78是首个源自MHV - 78感染宿主肿瘤的连续细胞系,其携带病毒基因组或至少其几个基因,并且仅在激活时极少产生感染性病毒。可以推测该细胞系主要与MHV - 78相关,并将成为研究MHV - 78潜伏性的宝贵工具。

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