Hrabovská Z, Chalupková A, Mistríková J
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic.
Acta Virol. 2010;54(1):55-60.
Murine herpesviruses 60 and 78 (MHV-60, MHV-78), closely related to Mouse herpesvirus strain 68 (MHV-68), are oncogenic lymphotropic gammaherpesviruses, which may serve as models for study of human oncogenic gammaherpesviruses such as Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV). In this work, we attempted to detect an analog of the MHV-68 ORF73 gene in tumors induced in mice either directly by MHV-60 or indirectly by MHV-78 via inoculation of NB-78 cells derived from a tumor induced by MHV-78. Besides the ORF73 gene, viral antigen and infectious virus were searched for. We succeeded in inducing lymphomas in mice by NB-78 cells and thus confirmed their transformed character. Importantly, we showed that the tumors induced by either MHV-60 or NB-78 cells were positive for the ORF73 gene, viral antigen and infectious virus. These results confirmed the generally accepted hypothesis about the connection between reactivation of latent gammaherpesviruses and malignant tumorigenesis.
鼠疱疹病毒60型和78型（MHV - 60、MHV - 78）与小鼠疱疹病毒68型毒株（MHV - 68）密切相关，是致癌性嗜淋巴细胞γ疱疹病毒，可作为研究人类致癌性γ疱疹病毒如爱泼斯坦 - 巴尔病毒（EBV）和卡波西肉瘤相关疱疹病毒（KSHV）的模型。在这项研究中，我们试图在由MHV - 60直接诱导或由MHV - 78通过接种源自MHV - 78诱导肿瘤的NB - 78细胞间接诱导的小鼠肿瘤中检测MHV - 68 ORF73基因的类似物。除了ORF73基因外，还对病毒抗原和传染性病毒进行了检测。我们成功地通过NB - 78细胞在小鼠中诱导出淋巴瘤，从而证实了它们的转化特性。重要的是，我们发现由MHV - 60或NB - 78细胞诱导的肿瘤中ORF73基因、病毒抗原和传染性病毒均呈阳性。这些结果证实了关于潜伏性γ疱疹病毒激活与恶性肿瘤发生之间联系的普遍接受的假说。
