Convenient method for monitoring Abeta aggregation by quartz-crystal microbalance.
作者信息
Okuno Hiroaki, Mori Kanae, Jitsukawa Tomofumi, Inoue Hiromi, Chiba Shinya
机构信息
Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1 Funabashi, Chiba 274-8510, Japan.
出版信息
Chem Biol Drug Des. 2006 Nov;68(5):273-5. doi: 10.1111/j.1747-0285.2006.00446.x.
Abstract
We have found that a quartz-crystal microbalance is a facile and useful tool for detecting the specific aggregation of the amyloid-beta peptides responsible for Alzheimer's disease. This method greatly reduces the time and samples (corresponds to 1 Hz frequency decrease per 30 pg mass increase on a 4.9 mm(2) electrode) that are needed in the aggregation assay, and is also applicable to molecular interactions between smaller amyloid-beta fragment peptides such as the pentapeptide KLVFF.
摘要
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