Ogawa Mayumi, Takamatsu Misako, Okamoto Manabu, Baba Hiroshi, Seo Kenji, Fujiwara Naoshi
Division of Anesthesiology, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-Dori, Niigata, Japan.
Neurosci Res. 2007 Mar;57(3):467-72. doi: 10.1016/j.neures.2006.11.011. Epub 2006 Dec 18.
To investigate plastic changes in nociceptive sensitivity of the dorsal horn, slow excitatory responses elicited by iteration of high-frequency stimulation were spatiotemporally observed in spinal cord slices of young-adult rats using membrane excitation imaging techniques. Single-pulse stimulation to the dorsal root elicited membrane excitation in lamina II, and high-frequency pulse-train stimulation evoked long-lasting excitation that expanded widely in the dorsal horn. Iteration of high-frequency stimulation enhanced the strength and extent of the excitatory responses, but such augmentation of the excitatory responses disappeared in the presence of an NMDA receptor antagonist (CPP) and was hindered by an NK1 receptor antagonist (L-703.606). The results suggest that activation of both NMDA and NK1 receptors is involved in the enhancement of slow excitatory responses evoked by iteration of high-frequency stimulation.
为了研究脊髓背角伤害性感受敏感性的可塑性变化，利用膜兴奋成像技术在成年幼鼠的脊髓切片中对高频刺激重复引发的缓慢兴奋性反应进行了时空观察。对背根进行单脉冲刺激可在Ⅱ层引发膜兴奋，高频脉冲串刺激可诱发在背角广泛扩展的持久兴奋。高频刺激的重复增强了兴奋性反应的强度和范围，但在NMDA受体拮抗剂（CPP）存在的情况下，这种兴奋性反应的增强消失了，并且受到NK1受体拮抗剂（L-703.606）的阻碍。结果表明，NMDA和NK1受体的激活均参与了高频刺激重复引发的缓慢兴奋性反应的增强。
