Teshima Reiko, Amano Fumio, Nakamura Ryosuke, Tanaka Yasuhiko, Sawada Jun-ichi
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences, 1-18-1 Kamiyoga, Tokyo, Japan.
Int Immunopharmacol. 2007 Feb;7(2):205-10. doi: 10.1016/j.intimp.2006.09.017. Epub 2006 Oct 23.
To investigate the biological activity of various polyunsaturated fatty acids (PUFAs) on the allergic reaction, we examined the effects of six PUFAs and two saturated fatty acids on calcium response and degranulation from rat basophilic leukemia (RBL-2H3) cells. Between 20 and 40 microM of six PUFAs (omega-6 series: arachidonic acid [AA, C20:4], gamma-linolenic acid [gamma-LN, C18:3] and linoleic acid [LA, C18:2]; omega-3 series: alpha-linolenic acids [alpha-LN, C18:3] and eicosapentaenoic acid [EPA, C20:5]; and omega-9 series: oleic acid [OLE, C18:1]), or two saturated fatty acids (stearic acid [STA, C18:0] and arachidic acid [AD, C20:0]) were used to examine the effects on calcium response and degranulation from RBL-2H3 cells. Calcium response was monitored using the fluorescent calcium indicator fura-2, while degranulation was monitored by measuring histamine release from the cells. Three omega-6 PUFAs (AA, alpha-LN and LA) dose-dependently increased the cytosolic free-calcium concentration and histamine release from RBL-2H3 cells. This phenomenon was specific to the omega-6 PUFAs, the omega-3 PUFAs (alpha-LA and EPA), omega-9 PUFA (OLE) and the saturated fatty acids (STA and AD) had no effect. The increase in the cytosolic free-calcium concentration caused by the omega-6 PUFAs depended on the existence of external calcium, cell viability and the cellular IP(3) levels remained unchanged throughout the experiment. These results suggest that omega-6 PUFAs work as direct mediators of calcium signaling pathways in RBL-2H3 cells.
为研究各种多不饱和脂肪酸（PUFAs）对过敏反应的生物活性，我们检测了六种PUFAs和两种饱和脂肪酸对大鼠嗜碱性白血病（RBL-2H3）细胞钙反应和脱颗粒的影响。使用20至40微摩尔的六种PUFAs（ω-6系列：花生四烯酸[AA，C20:4]、γ-亚麻酸[γ-LN，C18:3]和亚油酸[LA，C18:2]；ω-3系列：α-亚麻酸[α-LN，C18:3]和二十碳五烯酸[EPA，C20:5]；以及ω-9系列：油酸[OLE，C18:1]），或两种饱和脂肪酸（硬脂酸[STA，C18:0]和花生酸[AD，C20:0]）来检测对RBL-2H3细胞钙反应和脱颗粒的影响。使用荧光钙指示剂fura-2监测钙反应，同时通过测量细胞组胺释放来监测脱颗粒。三种ω-6 PUFAs（AA、α-LN和LA）剂量依赖性地增加RBL-2H3细胞的胞质游离钙浓度和组胺释放。这种现象是ω-6 PUFAs特有的，ω-3 PUFAs（α-LA和EPA）、ω-9 PUFA（OLE）和饱和脂肪酸（STA和AD）没有影响。ω-6 PUFAs引起的胞质游离钙浓度增加依赖于细胞外钙的存在，整个实验过程中细胞活力和细胞IP(3)水平保持不变。这些结果表明，ω-6 PUFAs在RBL-2H3细胞中作为钙信号通路的直接介质发挥作用。
