通过1H NMR和MALDI TOF质谱对亚麻纤维中组织特异性半乳聚糖的结构表征
[Structural characterization of tissue-specific galactan from flax fibers by 1H NMR and MALDI TOF mass spectrometry].
作者信息
Gur'ianov O, Gorshkova T, Kabel M, Schols H, van Dam J E G
出版信息
Bioorg Khim. 2006 Nov-Dec;32(6):621-31.
A high-molecular-mass polysaccharide galactan (M 2000 kDa) was isolated from flax at the stage of cell wall thickening of the bast fiber development. The polymer structure was studied by 1H NMR spectroscopy and MALDI TOF mass spectrometry. It is built up of Gal (59%), Rha (15%), GalA (23%), and Ara (3%) residues. The galactan backbone consists of successively alternating monomer disaccharide units (--> 4GalA1 --> 2Rha1 -->)n and is similar in its structure to the backbone of rhamnogalacturonan-1 (RG-I). Rhamnose residues bear in position 4 beta-(1 --> 4)-galactose side chains of various lengths with a polymerization degree of up to 28 or higher. A part of the side chains have branchings.
在韧皮纤维发育的细胞壁增厚阶段，从亚麻中分离出一种高分子量多糖半乳聚糖（M 2000 kDa）。通过1H NMR光谱和MALDI TOF质谱对聚合物结构进行了研究。它由Gal（59%）、Rha（15%）、GalA（23%）和Ara（3%）残基组成。半乳聚糖主链由连续交替的单体二糖单元（--> 4GalA1 --> 2Rha1 -->）n组成，其结构与鼠李糖半乳糖醛酸聚糖-1（RG-I）的主链相似。鼠李糖残基在4位带有不同长度的β-(1 --> 4)-半乳糖侧链，聚合度高达28或更高。部分侧链有分支。
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