Silipo Alba, Molinaro Antonio, Nazarenko Evgeny L, Sturiale Luisa, Garozzo Domenico, Gorshkova Raisa P, Nedashkovskaya Olga I, Lanzetta Rosa, Parrilli Michelangelo
Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, via Cintia 4, 80126 Napoli, Italy.
Carbohydr Res. 2005 Nov 21;340(16):2540-9. doi: 10.1016/j.carres.2005.08.021. Epub 2005 Oct 5.
The structure of the carbohydrate backbone of the lipooligosaccharide (LOS) of the marine bacterium Arenibacter certesii strain KMM 3941(T) has been elucidated. The structure was obtained by means of compositional analysis, matrix-assisted laser desorption/ionization mass spectrometry and complete 1H and 13C and 31P NMR spectroscopy. It shows novel and interesting aspects and is the first description of Arenibacter lipopolysaccharides. Strong and mild alkaline treatments, to fully deacylate and only to O-deacylate the LOS were performed in order to determine the core structure. The core consists of a mixture of species differing by the presence of a non-stoichiometric alpha-d-rhamnose residue. The Kdo unit is substituted at O-5 by alpha-mannose and at O-4 by a alpha-galactosyluronic acid phosphate. The lipid A is constituted by a bis-phosphorylated disaccharide unit composed by a 2,3-diamino-2,3-dideoxy-beta-d-glucopyranose (DAG) residue as non-reducing end and a GlcN as reducing end.
海洋细菌卡氏阿雷尼菌KMM 3941(T)脂寡糖（LOS）碳水化合物主链的结构已被阐明。该结构通过组成分析、基质辅助激光解吸/电离质谱以及完整的1H、13C和31P核磁共振光谱获得。它展现出新颖且有趣的方面，是对阿雷尼菌脂多糖的首次描述。为了确定核心结构，进行了强碱和弱碱处理，以使LOS完全脱酰基以及仅进行O-脱酰基。核心由因存在非化学计量的α-D-鼠李糖残基而不同的多种物质组成。Kdo单元在O-5位被α-甘露糖取代，在O-4位被α-半乳糖醛酸磷酸取代。脂质A由一个双磷酸化二糖单元构成，该单元由一个作为非还原端的2,3-二氨基-2,3-二脱氧-β-D-葡萄糖吡喃糖（DAG）残基和一个作为还原端的葡糖胺组成。
