Bae S Y, Lee C H, Kim J S, Lim C S, Lee C K, Lee K N, Park G H, Hur D S, Chung C, Chang J K
Department of Laboratory Medicine, Brain Korea 21 Program for Biomedical Science, Seoul, South Korea 136-707.
Vox Sang. 2007 Jan;92(1):64-8. doi: 10.1111/j.1423-0410.2006.00854.x.
The accurate determination of residual white blood cell (WBC) in blood components is of considerable clinical importance, and a variety of methods have been devised for the counting of low levels of residual WBC. In this study, we evaluated the performance of microscopic cell counter with microchannel plastic chip (C-reader) with regard to its ability to quantify WBC in WBC-reduced red cell concentrates.
In order to quantify residual WBC with the microscopic cell counter, WBC-reduced red cell concentrate was stained using propidium iodide. Three studies were performed: linearity, precision and correlation compared to those of manual Nageotte chamber counting and automatic flow cytometric methods.
Dilution experiments, conducted over a range of 0.7-712 WBC/microl, showed a linearity of r(2) > 0.999, with coefficient of variation values of < or = 15.6% and accuracy of 93.8% over all tested ranges. In comparison with the Nageotte chamber counting and flow cytometric methods, the correlation coefficients were r(2) > 0.995. The detection limit of this method was 0.24 WBC/microl. Total analysis time per sample was approximately 5 min.
The microscopic cell counter for residual WBC counting was determined to be efficient at the level of currently defined standards, with acceptable precision and accuracy. This method may prove useful for the quality assurance and control of WBC-depleted blood products.
准确测定血液成分中残留的白细胞（WBC）具有重要的临床意义，人们已设计出多种方法来计数低水平的残留白细胞。在本研究中，我们评估了带有微通道塑料芯片的显微镜细胞计数器（C-reader）在定量白细胞去除红细胞浓缩物中白细胞方面的性能。
为了用显微镜细胞计数器定量残留白细胞，用碘化丙啶对白细胞去除红细胞浓缩物进行染色。进行了三项研究：与手动纳盖特计数室计数和自动流式细胞术方法相比的线性、精密度和相关性。
在0.7 - 712个白细胞/微升的范围内进行的稀释实验显示，r(2) > 0.999的线性关系，在所有测试范围内变异系数值≤15.6%，准确度为93.8%。与纳盖特计数室计数和流式细胞术方法相比，相关系数为r(2) > 0.995。该方法的检测限为0.24个白细胞/微升。每个样品的总分析时间约为5分钟。
经测定，用于残留白细胞计数的显微镜细胞计数器在当前定义的标准水平上是有效的，具有可接受的精密度和准确度。该方法可能对白细胞去除血液制品的质量保证和控制有用。
