Koyama Koichi, Uobe Kenichi, Tanaka Akio
Department of Oral Pathology, Osaka Dental University, Hirakata, Osaka, Japan.
J Oral Pathol Med. 2007 Jan;36(1):18-24. doi: 10.1111/j.1600-0714.2006.00490.x.
Although human papillomavirus (HPV) infection has been shown to be a significant carcinogen in cervical squamous cell carcinoma (SCC), its significance in oral SCC remains unclear.
We developed highly sensitive detection methods for HPV to elucidate the prevalence and localization of HPV in paraffin sections from human oral SCC using modified in situ polymerase chain reaction (PCR) and in situ hybridization AT tailing (ISH-AT). Analyses revealed a high prevalence of several HPV types (HPV-16, -18, -22, -38 and -70) under optimal conditions. The ISH-AT method can be used as an alternative to in situ PCR.
Various staining patterns were observed in the 20 cases examined, and HPV-positive cells were localized within the surface epithelium as well as in neoplastic cells. We demonstrated that HPV-DNA could be detected in paraffin sections using either the method of in situ PCR or ISH, providing an appropriate primer and probe are used.
These results suggest that HPV infection could be one of several risk factors being involved in oral SCC.
尽管人乳头瘤病毒（HPV）感染已被证明是宫颈鳞状细胞癌（SCC）的一种重要致癌物，但其在口腔鳞状细胞癌中的意义仍不明确。
我们开发了用于HPV的高灵敏度检测方法，以利用改良的原位聚合酶链反应（PCR）和原位杂交加尾法（ISH-AT）阐明HPV在人口腔鳞状细胞癌石蜡切片中的患病率和定位。分析显示在最佳条件下几种HPV类型（HPV-16、-18、-22、-38和-70）的患病率很高。ISH-AT方法可作为原位PCR的替代方法。
在所检查的20例病例中观察到了各种染色模式，HPV阳性细胞定位于表面上皮以及肿瘤细胞内。我们证明，只要使用合适的引物和探针，就可以通过原位PCR或ISH方法在石蜡切片中检测到HPV-DNA。
这些结果表明HPV感染可能是参与口腔鳞状细胞癌的多种危险因素之一。
