Liu Chung-Ji, Lui Man-Tin, Chen Hsin-Lan, Lin Shu-Chun, Chang Kuo-Wei
School of Dentistry, National Yang-Ming University, Peitou, Taipei 112, Taiwan.
J Oral Pathol Med. 2007 Jan;36(1):43-7. doi: 10.1111/j.1600-0714.2006.00471.x.
The concentration of MICA in serum seems be a good candidate marker in cancer. Previous studies from our laboratory have shown that the polymorphic MIC gene may confer a risk for oral squamous cell carcinoma (OSCC). The study investigated the expression levels of MICA and MICB of OSCC patients and cancer cell lines.
We used RT-PCR to analyze the mRNA expression of MICA and MICB in four oral cancer cell lines compared with three normal human oral keratinocyte (NHOK) cell lines and in tissues from 36 patients with OSCC comparing tumor tissue with non-cancerous matched tissue (NCMT).
Endogenous MICB mRNA expression in OSCC cell lines was significantly higher than that in NHOK (1.40 +/- 0.27 vs. 0.40 +/- 0.16; P = 0.04). In 20 of 36 sets of tissue from patients with OSCC, MICB mRNA expression was higher in the cancerous tissue than in the NCMT. The mean MICB mRNA expression in OSCC tissues was significantly higher than in NCMT (0.39 +/- 0.08 vs. 0.14 +/- 0.03, P = 0.009, paired t-test). A significantly lower MICA mRNA was found in patients who chewed areca nut compared with those who did not use areca (P = 0.001) and in patients with well-differentiated tumors compared with those with less well-differentiated tumors (P = 0.02).
MICA and MICB mRNA expression may be increased in OSCC but there appears to be individual variation.
血清中MICA的浓度似乎是癌症的一个良好候选标志物。我们实验室之前的研究表明，多态性MIC基因可能会增加患口腔鳞状细胞癌（OSCC）的风险。本研究调查了OSCC患者和癌细胞系中MICA和MICB的表达水平。
我们使用逆转录聚合酶链反应（RT-PCR）分析了四种口腔癌细胞系中MICA和MICB的mRNA表达，并与三种正常人口腔角质形成细胞（NHOK）系进行比较，同时分析了36例OSCC患者的肿瘤组织和配对的非癌组织（NCMT）中的MICA和MICB的mRNA表达。
OSCC细胞系中内源性MICB mRNA表达显著高于NHOK细胞系（1.40±0.27对0.40±0.16；P = 0.04）。在36例OSCC患者的组织样本中，有20例癌组织中的MICB mRNA表达高于NCMT。OSCC组织中MICB mRNA的平均表达水平显著高于NCMT（0.39±0.08对0.14±0.03，P = 0.009，配对t检验）。与不嚼槟榔的患者相比，嚼槟榔的患者中MICA mRNA显著降低（P = 0.001），与低分化肿瘤患者相比，高分化肿瘤患者中MICA mRNA显著降低（P = 0.02）。
OSCC中MICA和MICB mRNA表达可能增加，但存在个体差异。
