Kim Hyun Jin, Kwon Min Sung, Choi Joon Sig, Kim Bo Hye, Yoon Jae Keun, Kim Kwan, Park Jong-Sang
School of Chemistry and Molecular Engineering, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Bioorg Med Chem. 2007 Feb 15;15(4):1708-15. doi: 10.1016/j.bmc.2006.12.004. Epub 2006 Dec 15.
Many therapeutic carrier materials were exploited for human gene therapy from viral to polymeric vectors. This research describes the evaluation of two biodegradable ester-bonded polymers synthesized by double-monomer polycondensation for a non-viral cationic polymer-based gene delivery system. The backbone was constructed to include inner tertiary amines and outer primary amines. Self-assembly with DNA resulted in the production of regularly nano-sized spherical polyplexes with good transfection efficiency, especially in the presence of serum. The polymers showed a relatively slow degradability for an amine-containing ester polymer, as they maintained DNA/polymer complex for 7 days in physiological buffer conditions. Finally, the low toxicity and slow degradation concluded these polymers reliable for long-term therapeutic applications.
从病毒载体到聚合物载体，人们开发了许多治疗性载体材料用于人类基因治疗。本研究描述了对通过双单体缩聚合成的两种可生物降解的酯键聚合物用于基于非病毒阳离子聚合物的基因递送系统的评估。主链构建为包含内部叔胺和外部伯胺。与DNA自组装产生了具有良好转染效率的规则纳米尺寸球形多聚体，尤其是在血清存在的情况下。对于含胺酯聚合物，这些聚合物显示出相对较慢的降解性，因为它们在生理缓冲条件下将DNA/聚合物复合物维持了7天。最后，低毒性和缓慢降解表明这些聚合物对于长期治疗应用是可靠的。
