脯氨酸-5-羧酸盐对小鼠肝脏中核糖-5-磷酸和5-磷酸核糖-1-焦磷酸生成的体内刺激作用：核糖-5-磷酸可用性在核苷酸合成中的调节作用的证据。

Stimulation of ribose-5-phosphate and 5-phosphoribosyl-1-pyrophosphate generation by pyrroline-5-carboxylate in mouse liver in vivo: evidence for a regulatory role of ribose-5-phosphate availability in nucleotide synthesis.

作者信息

Boer P, Sperling O

机构信息

Department of Clinical Biochemistry, Beilinson Medical Center, Petah-Tikva, Israel.

出版信息

Biochem Med Metab Biol. 1991 Aug;46(1):28-32. doi: 10.1016/0885-4505(91)90047-o.

DOI:10.1016/0885-4505(91)90047-o

PMID:1718342

Abstract

Pyrroline-5-carboxylase (P5C), a physiological stimulator of hexose-monophosphate-pentose pathway activity, was found before to increase 5-phosphoribosyl-1-pyrophosphate (PRPP) generation and nucleotide synthesis in human erythrocytes and cultured fibroblasts. We now report the stimulation of PRPP generation by P5C also in mouse liver in vivo. In addition we demonstrated a simultaneous elevation in ribose-5-phosphate (R5P) concentration, which was relatively smaller and transient. The demonstrated effect of P5C on liver R5P and PRPP content in vivo provides strong evidence for the physiological role of R5P availability in the regulation of PRPP and purine production.

摘要

吡咯啉 - 5 - 羧酸（P5C）是己糖单磷酸戊糖途径活性的生理刺激剂，之前发现它能增加人红细胞和培养的成纤维细胞中5 - 磷酸核糖 - 1 - 焦磷酸（PRPP）的生成及核苷酸合成。我们现在报告，P5C在体内也能刺激小鼠肝脏中PRPP的生成。此外，我们还证明了核糖 - 5 - 磷酸（R5P）浓度同时升高，但其升高幅度相对较小且是短暂的。P5C对体内肝脏R5P和PRPP含量的影响，为R5P可用性在调节PRPP和嘌呤生成中的生理作用提供了有力证据。