Bodeutsch C, de Wilde P C, van Houwelingen J C, Ebben G P, Kerstens H M, Kater L, van de Putte L B, Vooijs G P
Department of Pathology, University Hospital, Nijmegen, The Netherlands.
Anal Cell Pathol. 1991 Sep;3(5):299-310.
Recently two highly sensitive and specific diagnostic criteria for Sjögren's syndrome based on percentages of IgA-, IgG-, and IgM-containing plasma cells measured in immunohistologically stained labial salivary gland tissue have been described. The reliability of such a criterion is dependent on the accuracy, precision and inter-observer reproducibility in plasma cell counting. The present study evaluates the effect of tissue fixation and immunohistological procedures on the aforementioned factors. Immunoglobulin (Ig)-containing plasma cells in sections of lamellated submandibular salivary gland tissue, alternately fixed in a 4% buffered formol solution or formol-sublimate solution and stained with an indirect immunoperoxidase and unlabelled peroxidase anti-peroxidase (PAP) method respectively, were enumerated by three independent observers. Relative numbers of Ig-containing plasma cells appeared to be less sensitive for systematic errors due to tissue fixation and immunohistological procedure than absolute numbers of Ig-containing plasma cells. The best inter-observer reproducibility of plasma cell counts was obtained in sections from formol sublimate-fixed specimens stained according to the PAP procedure.
最近，有人描述了两种基于免疫组织化学染色的唇腺组织中含IgA、IgG和IgM浆细胞百分比的高度敏感和特异的干燥综合征诊断标准。这样一个标准的可靠性取决于浆细胞计数的准确性、精密度和观察者间的可重复性。本研究评估了组织固定和免疫组织学程序对上述因素的影响。分别用4%缓冲甲醛溶液或甲醛-升汞溶液交替固定、并用间接免疫过氧化物酶和未标记过氧化物酶抗过氧化物酶（PAP）方法染色的分层下颌下腺组织切片中的含免疫球蛋白（Ig）浆细胞，由三位独立观察者进行计数。相对于含Ig浆细胞的绝对数量，含Ig浆细胞的相对数量似乎对由于组织固定和免疫组织学程序导致的系统误差不太敏感。根据PAP程序染色的甲醛升汞固定标本切片获得了浆细胞计数的最佳观察者间可重复性。
