Gheuens J, Cras P, Perry G, Boons J, Ceuterick-de Groote C, Lübke U, Mercken M, Tabaton M, Gambetti P L, Vandermeeren M
Laboratory of Neuropathology, Born Bunge Foundation, University of Antwerp, Wilrijk, Belgium.
Brain Res. 1991 Aug 30;558(1):43-52. doi: 10.1016/0006-8993(91)90712-5.
A monoclonal antibody, termed NFT200, was raised after in vitro immunization with sonicated neurofibrillary tangle (NFT)-enriched fractions prepared from Alzheimer brain. The antigen to which NFT200 is directed was expressed in the paired helical filaments of NFT in sporadic and familial Alzheimer disease (AD), in the straight filaments of NFT in AD, progressive supranuclear palsy and of Pick bodies, and the NFT in several other conditions such as Parkinson-dementia complex of Guam and subacute sclerosing panencephalitis. Granulovacuolar degeneration of AD was also labeled with NFT200. Hirano bodies and amyloid deposits in AD, as well as Lewy bodies of idiopathic Parkinson disease lacked in the antigen. The NFT200-antigen was also expressed as a phosphatase-insensitive antigen in normal neurofilaments found in spinal cord and peripheral nerve axons but was absent from the perikaryal accumulation of neurofilaments induced by aluminum intoxication. Nevertheless, immunoblot studies failed to detect the NFT200 in isolated preparations of the neurofilament proteins, MAP-2, tau, ubiquitin or A4-amyloid peptide. The results indicate that the NFT200 monoclonal antibody is directed against a phosphatase-insensitive epitope of an axonal protein associated with neurofilaments but is labile to isolation and expressed as a stable epitope of a 200 kDa component of NFT.
一种名为NFT200的单克隆抗体，是在用从阿尔茨海默病大脑中制备的富含神经原纤维缠结（NFT）的超声破碎组分进行体外免疫后产生的。NFT200所针对的抗原在散发性和家族性阿尔茨海默病（AD）的NFT双螺旋丝中、AD的直丝中、进行性核上性麻痹和Pick小体中以及在其他几种病症（如关岛帕金森痴呆综合征和亚急性硬化性全脑炎）的NFT中表达。AD的颗粒空泡变性也被NFT200标记。AD中的 Hirano小体和淀粉样沉积物以及特发性帕金森病的路易小体缺乏该抗原。NFT200抗原在脊髓和周围神经轴突中的正常神经丝中也作为一种对磷酸酶不敏感的抗原表达，但在铝中毒诱导的神经丝核周聚集中不存在。然而，免疫印迹研究未能在神经丝蛋白、MAP - 2、tau、泛素或A4淀粉样肽的分离制剂中检测到NFT200。结果表明，NFT200单克隆抗体针对的是一种与神经丝相关的轴突蛋白的对磷酸酶不敏感的表位，但对分离不稳定，并且作为NFT 200 kDa组分的稳定表位表达。
