使用焦磷酸测序技术快速鉴定诺卡菌性角膜炎的菌种
Rapid species determination of Nocardia keratitis using pyrosequencing technology.
作者信息
Galor Anat, Hall Gerri S, Procop Gary W, Tuohy Marion, Millstein Michael E, Jeng Bennie H
机构信息
Cole Eye Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
出版信息
Am J Ophthalmol. 2007 Jan;143(1):182-3. doi: 10.1016/j.ajo.2006.07.036. Epub 2006 Aug 31.
PURPOSE
To describe a new technique, pyrosequencing, which allows for the rapid identification of Mycobacterium and Nocardia species.
DESIGN
Interventional case report.
METHODS
The medical records of a patient presenting with an infectious keratitis were reviewed.
RESULTS
A case of Nocardia abscessus/arthrititis/asiatica keratitis was diagnosed in a young individual with the aid of pyrosequencing technology. Based on presumed antibiotic sensitivities, therapy with topical trimethoprim-sulfamethoxazole eyedrops was initiated, and the infection was cleared rapidly with minimal residual scarring.
CONCLUSIONS
Pyrosequencing may be a useful tool in aiding the rapid diagnosis and treatment of ocular infections caused by slow-growing pathogens.
目的
描述一种新技术——焦磷酸测序，它可用于快速鉴定分枝杆菌属和诺卡菌属菌种。
设计
介入性病例报告。
方法
回顾了一名感染性角膜炎患者的病历。
结果
借助焦磷酸测序技术，一名年轻患者被诊断为脓肿诺卡菌/关节炎/亚洲变种角膜炎。根据推测的抗生素敏感性，开始局部使用甲氧苄啶-磺胺甲恶唑滴眼液进行治疗，感染迅速得到清除，仅留下最小程度的残余瘢痕。
结论
焦磷酸测序可能是辅助快速诊断和治疗由生长缓慢的病原体引起的眼部感染的有用工具。
Zotero 插件