Yin Xiaotang, Liang Shuang, Sun Xuguang, Luo Shiyun, Wang Zhiqun, Li Ran
Department of Ocular Microbiology, Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital University of Medical Science, Beijing, China.
Am J Ophthalmol. 2007 Oct;144(4):570-3. doi: 10.1016/j.ajo.2007.06.031. Epub 2007 Aug 15.
To assess hsp65 gene sequencing for detection and species identification of genus Nocardia from ocular isolates.
A prospective study based on laboratory investigation.
Genus-specific hsp65 gene sequencing was used to identify the genus Nocardia isolated from 11 consecutive cases of ocular nocardiosis to the species level.
Eleven eye clinical isolates belong to six species: N. arthritidis (3/11), N. neocaledoniensis (3/11), N. asiatica (2/11), N. asteroids type 4 (1/11), N. brasiliensis (1/11), and N. pseudobrasiliensis (1/11). N. arthritidis is the most important etiologic species that causes Nocardia keratitis. N. neocaledoniens is isolated in the conjunctiva, and might cause conjunctivitis.
Genus-specific hsp65 gene sequencing can be a rapid and reliable adjunct in the diagnosis of ocular Nocardia to the genus and species level. N. arthritidis other than N. asteroids (often appeared in surveys) is the most important etiologic species in ocular nocardiasis. N. neocaledoniensis can be isolated in conjunctiva, and might be able to cause conjunctivitis. The diversity of species has important implications for the etiology, pathogenesis, and treatment (drug susceptibility).
评估热休克蛋白65（hsp65）基因测序用于检测眼部分离的诺卡菌属及进行菌种鉴定的效果。
基于实验室研究的前瞻性研究。
采用属特异性hsp65基因测序将连续11例眼部诺卡菌病病例分离出的诺卡菌属鉴定到种水平。
11株眼部临床分离株分属于6个菌种：关节炎诺卡菌（3/11）、新喀里多尼亚诺卡菌（3/11）、亚洲诺卡菌（2/11）、4型星状诺卡菌（1/11）、巴西诺卡菌（1/11）和假巴西诺卡菌（1/11）。关节炎诺卡菌是引起诺卡菌性角膜炎的最重要病原体。新喀里多尼亚诺卡菌在结膜中分离得到，可能引起结膜炎。
属特异性hsp65基因测序可作为眼部诺卡菌属及种水平诊断的快速可靠辅助手段。除星状诺卡菌（常在调查中出现）外，关节炎诺卡菌是眼部诺卡菌病最重要的病原体。新喀里多尼亚诺卡菌可在结膜中分离得到，可能引起结膜炎。菌种的多样性对病因、发病机制和治疗（药敏性）具有重要意义。
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