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基于16S rRNA基因，通过全基因组扩增和微型凝胶-单链构象多态性技术对微生物群落进行分析。

16S rRNA gene-based analysis of microbial community by whole-genome amplification and minigel-single-strand conformation polymorphism technique.

作者信息

Oto Michiei, Suda Wataru, Shinoyama Hirofumi

机构信息

Department of Biotechnology, Tokyo Technical College, Higashi, Kunitachi-shi, Tokyo 186-0002, Japan.

出版信息

J Biosci Bioeng. 2006 Nov;102(5):482-4. doi: 10.1263/jbb.102.482.

DOI:10.1263/jbb.102.482
PMID:17189181
Abstract

We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.

摘要

我们已经开发出一种用于16S rRNA基因的分析技术，该技术包括全基因组扩增和聚合酶链反应（PCR）-微型凝胶-单链构象多态性技术（WGA-SSCP）。在最佳条件下，当来自目标细菌的基因组DNA占样本的0.5%或更多时，就可以检测到SSCP条带。该方法将有效地用于鉴定微生物群落中不可培养的细菌。

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