Synthesis of O-phosphotyrosine-containing peptides.

As a consequence of developments in peptide synthesis, it is now well established that Tyr(P)-containing peptides can be prepared in high yield by the use of Boc-Tyr(PO3Me2)-OH in Boc solution or solid-phase peptide synthesis or Fmoc-Tyr(PO3Me2)-OH in Fmoc/solid-phase peptide synthesis. It is considered that with further developments in solid-phase synthesis techniques and the use of alternative phosphate-protecting groups, the synthesis of large and complex Tyr(P)-containing peptides will be routine.