Kaito Chikara, Sekimizu Kazuhisa
Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 3-1, 7-Chome, Hongo, Tokyo 113-0033, Japan.
J Bacteriol. 2007 Mar;189(6):2553-7. doi: 10.1128/JB.01635-06. Epub 2006 Dec 28.
Wild-type Staphylococcus aureus rapidly expands on the surface of soft agar plates. The rates of expansion and the shapes of the resultant giant colonies were distinct for different strains of laboratory stocks and clinical isolates. The colony spreading abilities did not correlate with the biofilm-forming abilities in these strains. Insertional disruption of the dltABCD operon, which functions at the step of D-alanine addition to teichoic acids, and of the tagO gene, which is responsible for the synthesis of wall teichoic acids, decreased the colony spreading ability. The results indicate that wall teichoic acids and D-alanylation of teichoic acids are required for colony spreading.
野生型金黄色葡萄球菌在软琼脂平板表面迅速扩展。不同实验室菌株和临床分离株的扩展速率以及由此产生的巨大菌落的形状各不相同。在这些菌株中，菌落扩散能力与生物膜形成能力无关。参与磷壁酸D - 丙氨酸添加步骤的dltABCD操纵子以及负责壁磷壁酸合成的tagO基因的插入失活，降低了菌落扩散能力。结果表明，壁磷壁酸和磷壁酸的D - 丙氨酰化是菌落扩散所必需的。
