酸性和碱性成纤维细胞生长因子在大鼠、猴及人类黑质中的表达。
Expression of acidic and basic fibroblast growth factors in the substantia nigra of rat, monkey, and human.
作者信息
Bean A J, Elde R, Cao Y H, Oellig C, Tamminga C, Goldstein M, Pettersson R F, Hökfelt T
机构信息
Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden.
出版信息
Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10237-41. doi: 10.1073/pnas.88.22.10237.
The distribution of acidic (aFGF) and basic (bFGF) fibroblast growth factor mRNA and protein were examined in mesencephalon by immunohistochemistry, immunoblot analysis, in situ hybridization histochemistry, and RNA analysis. Coexistence of aFGF or bFGF with tyrosine hydroxylase protein in nigral cells was observed with immunohistochemistry. Both aFGF and bFGF mRNAs were found in the substantia nigra. Unilateral 6-hydroxydopamine lesions of nigrostriatal neurons resulted in a loss of aFGF and tyrosine hydroxylase [L-tyrosine, tetrahydropteridine: oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] mRNA-positive neurons on the lesioned side. The distribution of aFGF mRNA in monkey brain was similar to that seen in the rat. RNA and immunoblot analysis confirmed the presence of both aFGF and bFGF mRNAs and proteins in the substantia nigra of rat, monkey, and human.
通过免疫组织化学、免疫印迹分析、原位杂交组织化学和RNA分析，检测了中脑中酸性（aFGF）和碱性（bFGF）成纤维细胞生长因子mRNA和蛋白的分布。免疫组织化学观察到黑质细胞中aFGF或bFGF与酪氨酸羟化酶蛋白共存。在黑质中发现了aFGF和bFGF的mRNA。黑质纹状体神经元的单侧6-羟基多巴胺损伤导致损伤侧aFGF和酪氨酸羟化酶[L-酪氨酸，四氢生物蝶呤：氧氧化还原酶（3-羟化），EC 1.14.16.2]mRNA阳性神经元丢失。猴脑中aFGF mRNA的分布与大鼠相似。RNA和免疫印迹分析证实大鼠、猴和人的黑质中存在aFGF和bFGF的mRNA及蛋白。
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