Chin J F L, Chu J J H, Ng M L
Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597, Singapore.
Microbes Infect. 2007 Jan;9(1):1-6. doi: 10.1016/j.micinf.2006.09.009. Epub 2006 Dec 6.
Dengue virus (DV) is a flavivirus and its urban transmission is maintained largely by its mosquito vectors and vertebrate host, often human. In this study, investigation was carried out on the involvement of domain III of the envelope (E) glycosylated protein of dengue virus serotypes 1 and 2 (DV-1 and DV-2 DIII) in binding to host cell surfaces, thus mediating virus entry. Domain III protein of flavivirus can also serve as an attractive target in inhibiting virus entry. The respective DV DIII proteins were expressed as soluble recombinant fusion proteins before purification through enzymatic cleavage and affinity purification. The purified recombinant DV-1 and DV-2 DIII proteins both demonstrated the ability to inhibit the entry of DV-1 and DV-2 into HepG2 cells and C6/36 mosquito cells. As such, the DV DIII protein is indeed important for the interaction with cellular receptors in both human and mosquito cells. In addition, this protein induced antibodies that completely neutralized homologous dengue serotypes although not with the same efficiency among the heterologous serotypes. This observation may be of importance when formulating a generic vaccine that is effective against all dengue virus serotypes.
登革病毒（DV）是一种黄病毒，其在城市中的传播主要由蚊媒和脊椎动物宿主（通常是人类）维持。在本研究中，对登革病毒1型和2型（DV - 1和DV - 2）包膜（E）糖蛋白的结构域III（DV - 1和DV - 2 DIII）在与宿主细胞表面结合从而介导病毒进入过程中的作用进行了研究。黄病毒的结构域III蛋白也可作为抑制病毒进入的一个有吸引力的靶点。通过酶切和亲和纯化对各自的DV DIII蛋白进行纯化前，先将其表达为可溶性重组融合蛋白。纯化后的重组DV - 1和DV - 2 DIII蛋白均显示出能够抑制DV - 1和DV - 2进入HepG2细胞和C6/36蚊细胞。因此，DV DIII蛋白对于在人类和蚊细胞中与细胞受体的相互作用确实很重要。此外，该蛋白诱导产生的抗体能够完全中和同源登革血清型，尽管在异源血清型之间的中和效率不同。在制定针对所有登革病毒血清型均有效的通用疫苗时，这一观察结果可能具有重要意义。
