Martell R W, Arendse B, Jacobs M, Du Toit E D
Provincial Laboratory for Tissue Immunology, Cape Town, South Africa.
Tissue Antigens. 1991 Aug;38(2):95-8. doi: 10.1111/j.1399-0039.1991.tb01887.x.
DNA sequencing of HLA class II alleles has revealed a degree of polymorphism much greater than was expected on the basis of the standard serological typing methods. Amplification of the polymorphic second exon of the class II genes using the polymerase chain reaction, followed by hybridization with sequence-specific oligonucleotide probes, allows the unambiguous identification of alleles which could not be detected previously. Using the protocols of the Eleventh International Histocompatibility Workshop, we have applied this procedure for the typing of several individuals and their families with suspected alleles that had been observed using serology, cellular typing and restriction fragment length polymorphism (RFLPs). These included an allele related to DRw8 and DRw14, which has only been observed in the mixed ancestral South African population. In addition, unusual combinations of class II genes forming unique haplotypic associations were seen.
人类白细胞抗原（HLA）II类等位基因的DNA测序显示，其多态性程度比基于标准血清学分型方法预期的要高得多。使用聚合酶链反应扩增II类基因的多态性第二外显子，然后与序列特异性寡核苷酸探针杂交，能够明确鉴定出以前无法检测到的等位基因。按照第十一届国际组织相容性研讨会的方案，我们已将此方法应用于对几个个体及其家族进行分型，这些个体和家族具有通过血清学、细胞分型和限制性片段长度多态性（RFLP）观察到的疑似等位基因。其中包括一个与DRw8和DRw14相关的等位基因，该等位基因仅在具有混合祖先的南非人群中观察到。此外，还发现了形成独特单倍型关联的II类基因异常组合。
