Koh Sookyong, Magid Rhamy, Chung Hyokwon, Stine Christy D, Wilson Dawn N
Pediatrics, Children's Memorial Hospital, Northwestern University Feinburg School of Medicine Chicago, IL 60614, USA.
Epilepsy Behav. 2007 Feb;10(1):26-31. doi: 10.1016/j.yebeh.2006.11.008.
Depression is the most common psychiatric comorbidity in epilepsy. To better understand the contribution of seizures versus environment to depression in epilepsy, we investigated differential gene expression using microarray and quantitative RT-PCR, and depressive behavior, in the Porsolt forced swim test in juvenile rats reared in different environments after kainic acid (KA)-induced seizures. We selected for genes significantly down-regulated by KA seizures and upregulated by environmental enrichment. This common gene selection process yielded one known gene involved in mood and affect: serotonin receptor 5B. The changes in serotonin receptor gene expression were paralleled by decreased mobility in the forced swim tests; depressive behavior exhibited after seizures was no longer evident in rats reared in environmental enrichment. Our results suggest that seizures lead to increased susceptibility to depression through transcriptional regulation while environment, in turn, can interact with gene expression to influence the behavioral outcome of epilepsy.
抑郁症是癫痫中最常见的精神共病。为了更好地理解癫痫发作与环境对癫痫患者抑郁症的影响，我们在给予幼鼠海人酸（KA）诱导癫痫发作后，将其饲养在不同环境中，通过微阵列和定量逆转录聚合酶链反应研究差异基因表达，并在波索尔特强迫游泳试验中研究抑郁行为。我们筛选出在KA癫痫发作后显著下调且在环境富集后上调的基因。这一共同的基因筛选过程产生了一个已知的参与情绪和情感的基因：5B型血清素受体。血清素受体基因表达的变化与强迫游泳试验中活动能力的下降相平行；在环境富集环境中饲养的大鼠，癫痫发作后表现出的抑郁行为不再明显。我们的结果表明，癫痫发作通过转录调控导致对抑郁症的易感性增加，而环境反过来可以与基因表达相互作用，影响癫痫的行为结果。
