通过可逆还原电位测定DNA和RNA中嘌呤和嘧啶的绝对电子亲合势。
The determination of absolute electron affinities of the purines and pyrimidines in DNA and RNA from reversible reduction potentials.
作者信息
Wiley J R, Robinson J M, Ehdaie S, Chen E C, Chen E S, Wentworth W E
机构信息
Division of Science and Engineering, University of Texas, Odessa 79762.
出版信息
Biochem Biophys Res Commun. 1991 Oct 31;180(2):841-5. doi: 10.1016/s0006-291x(05)81141-6.
We report the reversible reduction potentials of the purines and pyrimidines of DNA and RNA. These were determined in dimethylsulfoxide using cyclic voltammetry. The absolute electron affinities have been determined from these reduction potentials by calibration with the absolute electron affinities for acridine and anthracene measured in the gas phase. These are the first experimentally determined values of the electron affinities of these purines and pyrimidines and are: Guanine = 1.51 eV, Adenine = 0.95 eV, Uracil = 0.80 eV, Thymine = 0.79 eV and Cytosine = 0.56 eV.
我们报告了DNA和RNA中嘌呤和嘧啶的可逆还原电位。这些电位是在二甲基亚砜中使用循环伏安法测定的。通过用气相中测定的吖啶和蒽的绝对电子亲和能进行校准，从这些还原电位确定了绝对电子亲和能。这些是这些嘌呤和嘧啶的电子亲和能的首批实验测定值，分别为：鸟嘌呤 = 1.51电子伏特，腺嘌呤 = 0.95电子伏特，尿嘧啶 = 0.80电子伏特，胸腺嘧啶 = 0.79电子伏特，胞嘧啶 = 0.56电子伏特。
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