Barendrecht M M, Chichester P, Michel M C, Levin R M
Department of Pharmacology and Pharmacotherapy, Academic Medical Center, Amsterdam, The Netherlands.
Auton Autacoid Pharmacol. 2007 Jan;27(1):47-53. doi: 10.1111/j.1474-8673.2006.00393.x.
1 The present study was designed to investigate the relationship between innervation density and contractile responses to field stimulation and exogenous agonists at early time points after induction of bladder outlet obstruction (BOO) in rats. 2 When compared with sham-operated animals, 1, 3 and 7 days of BOO were associated with a 75%, 80% and 90% increase of bladder weight. Field stimulation caused a frequency-dependent increase in force of contraction. The force of contraction was reduced at each frequency in BOO rats with the greatest decrease after 1 day and a gradual but incomplete recovery thereafter. In contrast, contractile responses to ATP, carbachol and KCl were markedly reduced after 1 day of BOO and fully recovered after 7 days. The neurofilament staining was not altered by 1 day of BOO, but gradually decreased with increasing duration of BOO reaching the lowest levels after 7 days. 3 We conclude that impaired cellular contractility seems to underlie the early reductions of field stimulation-induced contraction, possibly reflecting surgical trauma of the tissue. However, at later time points a reduced nerve density, possibly reflecting a partial denervation, appears to be the main reason for impaired contractile response to field stimulation.
1 本研究旨在探讨大鼠膀胱出口梗阻（BOO）诱导后早期，神经支配密度与对场刺激和外源性激动剂的收缩反应之间的关系。2 与假手术动物相比，BOO 1天、3天和7天时膀胱重量分别增加75%、80%和90%。场刺激引起收缩力的频率依赖性增加。BOO大鼠在各频率下收缩力均降低，1天后降低最为明显，此后逐渐但未完全恢复。相反，BOO 1天后对ATP、卡巴胆碱和氯化钾的收缩反应明显降低，7天后完全恢复。BOO 1天未改变神经丝染色，但随着BOO持续时间的增加逐渐减少，7天后达到最低水平。3 我们得出结论，细胞收缩功能受损似乎是场刺激诱导收缩早期降低的基础，可能反映了组织的手术创伤。然而，在后期，神经密度降低，可能反映了部分去神经支配，似乎是场刺激收缩反应受损的主要原因。
