[蜗牛蛋白和E-钙黏蛋白在胰腺癌中的表达及其临床意义]

[Expression of Snail and E-cadherin in pancreatic carcinoma and clinical significance thereof].

作者信息

Yin Tao, Wang Chun-you, Liu Tao, Zhao Gang, Zha Yun-hong

机构信息

Centre of Pancreas Surgery, Union Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan 430022, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2006 Oct 31;86(40):2821-5.

PMID:17200014

Abstract

OBJECTIVE

To investigate the expression of Snail, a newly discovered inhibitory transcription factor, and E-cadherin in pancreatic carcinoma (PC) and clinical significance thereof.

METHODS

Immunohistochemistry was used to examine the expression of Snail and E-cadherin in 56 specimens of PC obtained during operation. PC cells of the line of Panc-1 and transfected with pCMV-Tag2B-Snail, a eucaryotic expression vector coding Snail, so as to obtain a stable clone named Panc-1-Snail. RT-PCR was used to detect the mRNA expression of Snail gene in the Panc-1-Snail cells. Immunofluorescence technique and Western blotting were used to detect the expression of E-cadherin in the Pan-1-Snail cells. Transwell cell culture chamber method was used to examine the invasion ability of the Panc-1-and Panc-1-Snail cells.

RESULTS

Twenty of the 56 PC specimens (36%) were positive in Snail, mainly located in the nuclei. Seventeen of the 20 Snail-positive PC specimens were with and 3 without lymph node metastasis (P = 0.006); and 7 of the 8 PC specimens with lymph node metastasis were Snail-positive and only 1 was Snail-negative (P = 0.001). Twenty-six PC specimens showed low expression of E-cadherin with remarkable heterogeneity. Of the 20 Snail-positive PC specimens 13 showed low expression of E-cadherin and only 7 showed normal expression of E-cadherin; and 13 of the 36 Snail-negative PC specimens showed low expression of E-cadherin (P = 0.038). RT-PCR showed strong Snail mRNA expression in the Pan-1 cells. Both Western blotting and immunofluorescence technique showed the depression of E-cadherin expression. The Transwell chamber test showed that the number of penetrating Pan-1-Snail cells after 24-hour incubation was (174 +/- 8)/200X field of view, significantly greater than that of the Pan-1 cells [(96 +/- 4)/200X field of view, P < 0.01].

CONCLUSION

Re-expressed in PC and accelerating the invasion of PC through depressing E-cadherin expression, Snail may present a new marker for predicting the aggressiveness of PC.

摘要

目的

探讨新发现的抑制性转录因子Snail及E-钙黏蛋白在胰腺癌（PC）中的表达及其临床意义。

方法

采用免疫组织化学法检测56例手术切除的PC标本中Snail和E-钙黏蛋白的表达。用编码Snail的真核表达载体pCMV-Tag2B-Snail转染Panc-1系PC细胞，获得稳定克隆Panc-1-Snail。用RT-PCR检测Panc-1-Snail细胞中Snail基因的mRNA表达。用免疫荧光技术和蛋白质印迹法检测Pan-1-Snail细胞中E-钙黏蛋白的表达。采用Transwell细胞培养小室法检测Panc-1和Panc-1-Snail细胞的侵袭能力。

结果

56例PC标本中20例（36%）Snail阳性，主要位于细胞核。20例Snail阳性的PC标本中，17例有淋巴结转移，3例无淋巴结转移（P = 0.006）；8例有淋巴结转移的PC标本中，7例Snail阳性，仅1例Snail阴性（P = 0.001）。26例PC标本E-钙黏蛋白表达低，异质性明显。20例Snail阳性的PC标本中，13例E-钙黏蛋白表达低，仅7例E-钙黏蛋白表达正常；36例Snail阴性的PC标本中，13例E-钙黏蛋白表达低（P = 0.038）。RT-PCR显示Panc-1细胞中Snail mRNA表达强。蛋白质印迹法和免疫荧光技术均显示E-钙黏蛋白表达降低。Transwell小室试验显示，孵育24小时后，穿过Panc-1-Snail细胞的数量为（174±8）/200倍视野，明显多于Panc-1细胞[（96±4）/200倍视野，P < 0.01]。