Cox T, Campbell P, Peterson R N
Department of Physiology, School of Medicine, Southern Illinois University, Carbondale 62901.
Mol Reprod Dev. 1991 Oct;30(2):135-47. doi: 10.1002/mrd.1080300210.
Plasma membranes isolated from cauda epididymal and ejaculated boar sperm were inserted into planar lipid bilayers and examined for the presence of ion channels. Channel fusion was frequently observed; the most prominent was a nonselective cation channel which conducted K, Na, Cs, Ca, and Ba. Channel opening did not show a strict dependence on voltage but was partially blocked by verapamil, nitrendipine, and ruthenium red. A channel with these characteristics was observed when plasma membranes were isolated by high-pressure nitrogen cavitation (650 psi, 78% sperm head plasma membranes) or at very low nitrogen pressures (50 psi, 90% sperm head plasma membranes), suggesting that this channel may be present in the plasma membrane overlying the sperm head.
从附睾尾和射出的公猪精子中分离出的质膜被插入平面脂质双分子层中，并检测是否存在离子通道。经常观察到通道融合；最突出的是一种非选择性阳离子通道，它能传导钾、钠、铯、钙和钡。通道开放并不严格依赖于电压，但会被维拉帕米、尼群地平和钌红部分阻断。当通过高压氮气空化（650磅力/平方英寸，78%精子头部质膜）或在非常低的氮气压力（50磅力/平方英寸，90%精子头部质膜）下分离质膜时，观察到具有这些特征的通道，这表明该通道可能存在于覆盖精子头部的质膜中。
