蛋白质磷酸酶亲和基质微囊藻毒素-琼脂糖凝胶和微囊藻毒素-生物素-琼脂糖凝胶的合成与应用。
Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose.
作者信息
Moorhead Greg B G, Haystead Timothy A J, MacKintosh Carol
机构信息
Department of Biological Sciences, University of Calgary, Alberta, Canada.
出版信息
Methods Mol Biol. 2007;365:39-45. doi: 10.1385/1-59745-267-X:39.
PMID:17200552
Abstract
Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.
摘要
基于微囊藻毒素的亲和基质已被用于证明信号蛋白与蛋白磷酸酶的关联，以及用于纯化低丰度的微囊藻毒素敏感蛋白磷酸酶。在此，我们描述了微囊藻毒素-琼脂糖凝胶和微囊藻毒素-生物素-琼脂糖凝胶的合成及使用方法。
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