具有激发态分子内质子转移特性的蛋白质探针：一种在淀粉提取过程中检测水稻蛋白质的灵敏方法。
ESIPT-exhibiting protein probes: a sensitive method for rice proteins detection during starch extraction.
作者信息
Cardoso Mateus Borba, Samios Dimitrios, da Silveira Nádya Pesce, Rodembusch Fabiano Severo, Stefani Valter
机构信息
Universidade Federal do Rio Grande do Sul-Instituto de Química, Laboratório de Instrumentação e Dinâmica Molecular, Av. Bento Gonçalves, 9500. CP 15003 CEP 91501-970, Porto Alegre-RS, Brazil.
出版信息
Photochem Photobiol Sci. 2007 Jan;6(1):99-102. doi: 10.1039/b610999c. Epub 2006 Nov 2.
The 2-(4'-isothiocyanate-2'-hydroxyphenyl)benzoxazole dye was successfully applied as label of rice proteins during the alkaline extraction of starch. Direct fluorescence measurements were used to observe the presence of proteins labelled in different steps of rice starch extraction. The results were compared to those obtained with the well-known biuret colorimetric test. Whereas the colorimetric test indicates the absence of protein after the third extraction step, the fluorescence emission of the conjugate could be observed in all extraction steps. The separation of different rice proteins could also be observed.
2-(4'-异硫氰酸酯-2'-羟基苯基)苯并恶唑染料在淀粉碱性提取过程中成功用作大米蛋白的标记物。采用直接荧光测量法观察大米淀粉提取不同步骤中标记蛋白的存在情况。将结果与通过著名的双缩脲比色法获得的结果进行比较。虽然比色法表明在第三次提取步骤后不存在蛋白质，但在所有提取步骤中都能观察到共轭物的荧光发射。还可以观察到不同大米蛋白的分离情况。
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