Kanoh Yuhsaku, Ohara Tadashi, Mashiko Takaomi, Abe Tadashi, Masuda Noriyuki, Akahoshi Tohru
Department of Laboratory Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan.
Anticancer Res. 2006 Nov-Dec;26(6B):4293-7.
It has been demonstrated that increased expression of the sialyl Le(x) sugar chains on cancer cells influences cellular adhesion to vascular-endothelial cells. Therefore, it was thought that alterations in the sugar chain structure of E-cadherin, a calcium dependent adhesion molecule, influence the metastasis of cancer cells.
In this study, N-linked oligosaccharides of human serum immunoglobulin G (IgG) were analyzed in 12 patients with non-small cell lung cancer (NSCLC) (6 localized cancer: 3 adenocarcinomas and 3 squamous cell carcinomas; 6 metastatic cancer: 3 adenocarcinomas and 3 squamous cell carcinomas) and 10 healthy controls using fluorophore-associated carbohydrate electrophoresis (FACE). The relationship between changes in sugar chain structure and serum concentrations of carcinoembryonic antigen (CEA) and cytokeratin 19 fragment (CYFRA21-1) were evaluated. CEA levels in the sera were determined using an enzyme immunoassay, and CYFRA21-1 levels were determined using an enzyme chemiluminescent immunoassay.
Fri (monogalactosyl IgG oligosaccharides) and Fr2 (digalactosyl IgG oligosaccharides) decreased, while Fr4 (agalactosyl IgG oligosaccharides) significantly increased (p < 0.01-0.05) with NSCLC progression. The Fr4/Fr1+Fr2 ratio increased with NSCLC progression, and the ratios in localized and metastatic NSCLC were significantly higher than in healthy controls (p < 0.01 and p < 0.01, respectively). There was a strong correlation between serum CEA levels and Fr4 (r = 0.91) and a significant correlation between serum CEA levels and the Fr4/Fr1+Fr2 ratio (r = 0.83, p < 0.05) in patients with lung adenocarcinoma. There was a significant correlation between serum CYFRA21-1 levels and Fr4 (r = 0.88, p < 0.001) and a positive correlation between serum CYFRA21-1 levels and the Fr4/Fr1+Fr2 ratio (r = 0.38) in patients with lung squamous cell carcinoma.
The analysis of serum IgG N-linked oligosaccharide chain structures by FACE may be an auxiliary indicator of serum tumor markers for monitoring NSCLC progression.
已证实癌细胞上唾液酸化Le(x)糖链表达增加会影响细胞与血管内皮细胞的黏附。因此，人们认为钙依赖性黏附分子E-钙黏蛋白糖链结构的改变会影响癌细胞的转移。
在本研究中，使用荧光团相关碳水化合物电泳（FACE）分析了12例非小细胞肺癌（NSCLC）患者（6例局限性癌：3例腺癌和3例鳞状细胞癌；6例转移性癌：3例腺癌和3例鳞状细胞癌）和10名健康对照者血清免疫球蛋白G（IgG）的N-连接寡糖。评估了糖链结构变化与癌胚抗原（CEA）和细胞角蛋白19片段（CYFRA21-1）血清浓度之间的关系。血清CEA水平采用酶免疫法测定，CYFRA21-1水平采用酶化学发光免疫法测定。
随着NSCLC病情进展，Fri（单半乳糖基IgG寡糖）和Fr2（双半乳糖基IgG寡糖）减少，而Fr4（无半乳糖基IgG寡糖）显著增加（p<0.01-0.05）。随着NSCLC病情进展，Fr4/Fr1+Fr2比值增加，局限性和转移性NSCLC患者的该比值显著高于健康对照者（分别为p<
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