Su Chin-Cheng, Lin Jaung-Geng, Li Te-Mao, Chung Jing-Gung, Yang Jai-Sing, Ip Siu-Wan, Lin Wen-Chuan, Chen Guang-Wei
School of Chinese Medicine, Department of Microbiology, China Medical University, No 91, Hsueh-Shih Road, Taichung City 404, Taiwan, ROC.
Anticancer Res. 2006 Nov-Dec;26(6B):4379-89.
Curcumin (diferuloylmethane), the yellow pigment in turmeric (Curcuma longa), is known to inhibit proliferation of cancer cells by arresting them at various phases of the cell cycle and to induce apoptosis in tumor cells. Curcumin-induced apoptosis mainly involves the activation of caspase-3 and mitochondria-mediated pathway in various cancer cells of different tissue origin. In the present study, the induction of apoptosis and cytotoxicity by curcumin in colon cancer colo 205 cells was investigated by using flow cytometry. The results demonstrated that curcumin induced cytotoxicity and apoptosis dose- and time-depedently. Curcumin induced the production of reactive oxygen species (ROS) and Ca+2, decreased the levels of mitochondria membrane potential and induced caspase-3 activity. Curcumin also promoted the expression of Bax, cytochrome C, p53 and p21 but inhibited the expression of Bcl-2. These observations suggest that curcumin may have a possible therapeutic potential in colon cancer patients.
姜黄素（二阿魏酰甲烷）是姜黄（Curcuma longa）中的黄色色素，已知它可通过使癌细胞停滞于细胞周期的各个阶段来抑制癌细胞增殖，并诱导肿瘤细胞凋亡。姜黄素诱导的凋亡主要涉及不同组织来源的各种癌细胞中caspase-3的激活和线粒体介导的途径。在本研究中，通过流式细胞术研究了姜黄素对结肠癌colo 205细胞凋亡和细胞毒性的诱导作用。结果表明，姜黄素剂量和时间依赖性地诱导细胞毒性和凋亡。姜黄素诱导活性氧（ROS）和Ca+2的产生，降低线粒体膜电位水平并诱导caspase-3活性。姜黄素还促进Bax、细胞色素C、p53和p21的表达，但抑制Bcl-2的表达。这些观察结果表明，姜黄素可能对结肠癌患者具有潜在的治疗潜力。
