白细胞介素-6对人结肠直肠癌侵袭性的影响。

Influence of interleukin-6 on the invasiveness of human colorectal carcinoma.

作者信息

Hsu Chi-Pin, Chung Yuan-Chiang

机构信息

Department of Medical Technology, Yuanpei University, Taiwan, ROC.

出版信息

Anticancer Res. 2006 Nov-Dec;26(6B):4607-14.

PMID:17201185

Abstract

BACKGROUND

Plasma interleukin-6 (IL-6) level correlates with patient survival in colorectal carcinoma and is regarded as a prognostic factor. In this study, the role of IL-6 in colorectal carcinoma proliferation, chemotaxis and invasion was investigated.

MATERIALS AND METHODS

Proliferation and invasion were measured in four colorectal carcinoma cell lines. The effect of IL-6 (10, 50, 100 ng/ml) on progression (the ability to grow, adhere, chemotax and invade) was also measured.

RESULTS

Physiological (10 ng/ml) and pharmacological (50 and 100 ng/ml) concentrations of IL-6 did not significantly affect growth. However, IL-6 (10 ng/ml) significantly increased attachment to basement membrane (p < 0.05). IL-6 (50 ng/ml) significantly increased the chemotaxis, anchorage-independent growth and invasiveness of SW-480 (one of the four cell lines) (p < 0.05). IL-6 (100 ng/ml) resulted in negative feedback inhibition of these effects.

CONCLUSION

IL-6 plays an important role in the progression of colorectal carcinoma.

摘要

背景

血浆白细胞介素-6（IL-6）水平与结直肠癌患者的生存率相关，被视为一种预后因素。在本研究中，对IL-6在结直肠癌增殖、趋化性和侵袭中的作用进行了研究。

材料与方法

在四种结直肠癌细胞系中检测增殖和侵袭情况。还检测了IL-6（10、50、100 ng/ml）对进展（生长、黏附、趋化和侵袭能力）的影响。

结果

生理浓度（10 ng/ml）和药理浓度（50和100 ng/ml）的IL-6对生长无显著影响。然而，IL-6（10 ng/ml）显著增加了对基底膜的黏附（p < 0.05）。IL-6（50 ng/ml）显著增加了SW-480（四种细胞系之一）的趋化性、非锚定依赖性生长和侵袭性（p < 0.05）。IL-6（100 ng/ml）导致这些效应的负反馈抑制。