• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

睾丸酮假单胞菌中羟基类固醇脱氢酶对吗啡的酶促转化。

Enzymatic transformation of morphine by hydroxysteroid dehydrogenase from Pseudomonas testosteroni.

作者信息

Liras P, Kasparian S S, Umbreit W W

出版信息

Appl Microbiol. 1975 Oct;30(4):650-6. doi: 10.1128/am.30.4.650-656.1975.

DOI:10.1128/am.30.4.650-656.1975
PMID:172013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC187243/
Abstract

Eznyme preparations from Pseudomonas testosteroni containing alpha- and beta- hydroxysteroid dehydrogenases catalyzed the oxidation of morphine and codeine by nicotinamide adenine dinucleotide. Morphine was converted in relatively low yield into 14-hydroxymorphinone probably via morphinone as an intermediate. Codeine was converted to codeinone and 14-hydroxycodeinone. Only the conversions at the 6-position were carred out by the hydroxysteroid dehydrogenase. Hydroxylation at the 14-position did occur spontaneously (or enzymatically with a contaminating enzyme) ater oxidation at the 6-position.

摘要

来自睾丸酮假单胞菌的含有α-和β-羟基类固醇脱氢酶的酶制剂催化了烟酰胺腺嘌呤二核苷酸对吗啡和可待因的氧化反应。吗啡可能经吗啡酮作为中间体以相对较低的产率转化为14-羟基吗啡酮。可待因转化为可待因酮和14-羟基可待因酮。只有6位的转化是由羟基类固醇脱氢酶进行的。14位的羟基化在6位氧化后确实会自发发生（或由一种污染酶酶促发生）。

相似文献

1
Enzymatic transformation of morphine by hydroxysteroid dehydrogenase from Pseudomonas testosteroni.睾丸酮假单胞菌中羟基类固醇脱氢酶对吗啡的酶促转化。
Appl Microbiol. 1975 Oct;30(4):650-6. doi: 10.1128/am.30.4.650-656.1975.
2
Microbial degradation of the morphine alkaloids: identification of morphine as an intermediate in the metabolism of morphine by Pseudomonas putida M10.吗啡生物碱的微生物降解：证实吗啡是恶臭假单胞菌M10代谢吗啡过程中的一种中间产物。
Arch Microbiol. 1990;154(5):465-70. doi: 10.1007/BF00245229.
3
On the 3alpha-hydroxysteroid dehydrogenase from Pseudomonas testosteroni. The effect of denaturing agents.
Int J Pept Protein Res. 1975;7(4):335-9. doi: 10.1111/j.1399-3011.1975.tb02449.x.
4
On the 3 alpha-hydroxysteroid dehydrogenase from Pseudomonas testosteroni. Purification and properties.
Eur J Biochem. 1974 Jul 1;46(1):117-25. doi: 10.1111/j.1432-1033.1974.tb03603.x.
5
3Alpha-hydroxysteroid dehydrogenase from Pseudomonas testosteroni: kinetic properties with NAD and its thionicotinamide analogue.
Eur J Biochem. 1975 Jan 15;50(3):603-9. doi: 10.1111/j.1432-1033.1975.tb09901.x.
6
3(17)beta-Hydroxysteroid Dehydrogenase of Pseudomonas testosteroni. Ligand binding properties.
J Biol Chem. 1977 Jun 10;252(11):3784-90.
7
Reversibility of steroid delta-isomerase. 3. The soluble enzyme of Pseudomonas testosteroni.
J Biol Chem. 1966 Jul 10;241(13):3154-7.
8
Metabolism of dexamethasone in the human kidney: nicotinamide adenine dinucleotide-dependent 11beta-reduction.地塞米松在人肾脏中的代谢：烟酰胺腺嘌呤二核苷酸依赖性11β-还原反应
J Clin Endocrinol Metab. 1997 May;82(5):1598-602. doi: 10.1210/jcem.82.5.3936.
9
Enzymatic determination of bile acids. The presence of a new enzyme, a 12alpha-hydroxysteroid: NAD-oxidoreductase in extracts from Pseudomonas testosteroni.
Scand J Gastroenterol. 1974;9(6):555-7.
10
Beta-hydroxysteroid dehydrogenase: activity in microemulsion and extraction from Pseudomonas testosteroni cells with microemulsion.
Biochimie. 1990 Apr;72(4):285-9. doi: 10.1016/0300-9084(90)90085-u.

引用本文的文献

1
Potential of the strain Raoultella sp. KDF8 for removal of analgesics.拉乌尔菌属菌株KDF8去除镇痛药的潜力
Folia Microbiol (Praha). 2018 May;63(3):273-282. doi: 10.1007/s12223-017-0563-2. Epub 2017 Nov 11.
2
Transformation of 2,2'-bimorphine to the novel compounds 10-alpha-S-monohydroxy-2,2'-bimorphine and 10,10'-alpha,alpha'-S,S'-dihydroxy-2,2'-bimorphine by Cylindrocarpon didymum.柱孢属真菌将2,2'-双吗啡转化为新型化合物10-α-S-单羟基-2,2'-双吗啡和10,10'-α,α'-S,S'-二羟基-2,2'-双吗啡。
Appl Environ Microbiol. 2001 Aug;67(8):3716-9. doi: 10.1128/AEM.67.8.3716-3719.2001.
3
Biological synthesis of the analgesic hydromorphone, an intermediate in the metabolism of morphine, by Pseudomonas putida M10.恶臭假单胞菌M10对镇痛剂氢吗啡酮（吗啡代谢中间体）的生物合成。
Appl Environ Microbiol. 1993 Jul;59(7):2166-70. doi: 10.1128/aem.59.7.2166-2170.1993.
4
Identification of transformation products arising from bacterial oxidation of codeine by Streptomyces griseus.由灰色链霉菌对可待因进行细菌氧化产生的转化产物的鉴定。
Appl Environ Microbiol. 1985 Oct;50(4):831-6. doi: 10.1128/aem.50.4.831-836.1985.
5
Microbial degradation of the morphine alkaloids. Purification and characterization of morphine dehydrogenase from Pseudomonas putida M10.吗啡生物碱的微生物降解。恶臭假单胞菌M10中吗啡脱氢酶的纯化与特性分析。
Biochem J. 1991 Mar 15;274 ( Pt 3)(Pt 3):875-80. doi: 10.1042/bj2740875.
6
Microbial degradation of the morphine alkaloids: identification of morphine as an intermediate in the metabolism of morphine by Pseudomonas putida M10.吗啡生物碱的微生物降解：证实吗啡是恶臭假单胞菌M10代谢吗啡过程中的一种中间产物。
Arch Microbiol. 1990;154(5):465-70. doi: 10.1007/BF00245229.

本文引用的文献

1
Crystalline alcohol dehydrogenase from baker's yeast.来自面包酵母的结晶乙醇脱氢酶。
J Biol Chem. 1950 May;184(1):313-9.
2
CRYSTALLIZATION OF NICOTINAMIDE ADENINE DINUCLEOTIDE.
J Biol Chem. 1964 Oct;239:PC3598+.
3
PHYSICAL AND CHEMICAL CHARACTERIZATION OF HYDROXYSTEROID DEHYDROGENASES FROM PSEUDOMONAS TESTOSTERONI.睾丸酮假单胞菌中羟基类固醇脱氢酶的物理和化学特性
Biochim Biophys Acta. 1964 Sep 18;89:409-21. doi: 10.1016/0926-6569(64)90067-7.
4
PURIFICATION OF STEROID-INDUCED ENZYMES FROM PSEUDOMONAS TESTOSTERONI.从睾丸酮假单胞菌中纯化甾体诱导酶
Biochim Biophys Acta. 1964 Sep 18;89:398-408. doi: 10.1016/0926-6569(64)90066-5.
5
The purification of nicotinamide adenine dinucleotide and kinetic effects of nucleotide impurities.烟酰胺腺嘌呤二核苷酸的纯化及核苷酸杂质的动力学效应
J Biol Chem. 1963 Apr;238:1538-43.
6
Specificity, kinetics, and inhibition of alpha- and beta-hydroxysteroid dehydrogenases.
J Biol Chem. 1956 Feb;218(2):675-91.
7
Induction and purification of alpha- and beta-hydroxysteroid dehydrogenases.α-和β-羟基类固醇脱氢酶的诱导与纯化
J Biol Chem. 1956 Feb;218(2):661-74.
8
Enzymatic formation of 3 alpha-hydroxysteroids.
Nature. 1954 Jun 19;173(4416):1189-90. doi: 10.1038/1731189a0.
9
Purification and properties of a beta-hydroxysteroid dehydrogenase.一种β-羟基类固醇脱氢酶的纯化及性质
J Biol Chem. 1953 Dec;205(2):823-37.
10
Purification and substrate specificities of bacterial hydroxysteroid dehydrogenases.
Biochemistry. 1969 Dec;8(12):5093-103. doi: 10.1021/bi00840a063.