Di Cara F, Morra R, Cavaliere D, Sorrentino A, De Simone A, Polito C L, Digilio A F
Institute of Genetics and Biophysics Adriano Buzzati Traverso, Via P. Castellino 111, Naples, Italy.
Insect Mol Biol. 2006 Dec;15(6):813-22. doi: 10.1111/j.1365-2583.2006.00688.x.
We report the characterization of two novel genes of Drosophila melanogaster, named mst36Fa and mst36Fb. They define a novel gene family, showing identical time and tissue-specificity limited to male germ cells where their transcription starts during meiotic prophase. These two genes encode for two slightly basic proteins highly homologous to each other and fairly rich in leucine and glutamic acid. Although strictly clustered, these genes utilize different promoter regions as revealed by examination of transgenic flies bearing mst36F-promoter-lacZ reporter constructs and by reverse transcription-polymerase chain reaction assays. Our data suggest that at least one gene (mst36Fa) of the cluster is under translational repression until spermiogenesis suggesting a putative role in the spermatides differentiation. The present study is aimed at the structural analysis of these genes.
我们报道了黑腹果蝇两个新基因mst36Fa和mst36Fb的特征。它们定义了一个新的基因家族，具有相同的时间和组织特异性，仅限于雄性生殖细胞，其转录在减数分裂前期开始。这两个基因编码两种彼此高度同源且富含亮氨酸和谷氨酸的微碱性蛋白质。尽管紧密聚集，但通过检查携带mst36F启动子 - lacZ报告构建体的转基因果蝇以及逆转录 - 聚合酶链反应分析表明，这些基因利用不同的启动子区域。我们的数据表明，该基因簇中至少有一个基因（mst36Fa）在精子发生之前处于翻译抑制状态，这表明其在精子细胞分化中可能发挥作用。本研究旨在对这些基因进行结构分析。
