Di Felice G, Tinghino R, Federico R, Afferni C, Liberatore P, Pini C
Laboratorio di Immunologia, Istituto Superiore di Sanità, Rome, Italy.
Ann Ist Super Sanita. 1991;27(1):183-7.
The diagnosis of allergic diseases is commonly carried out by using poorly standardized preparations containing allergenic and non-allergenic components. Recently, monoclonal antibody technology has been applied to the biochemical characterization and to the purification of allergens, in order to evaluate their potential role in the standardization of diagnostic and therapeutic extracts. Three main results have been achieved: 1) the characterization of allergenic epitopes recognized by IgE and IgG from patients' sera; 2) the purification by affinity chromatography of relevant allergenic components; 3) the development of immunoassays for the quantitation of allergens in the commercially available extracts used for diagnosis and therapy.
过敏性疾病的诊断通常使用含有过敏原和非过敏原成分、标准化程度较低的制剂来进行。最近，单克隆抗体技术已应用于过敏原的生化特性分析和纯化，以评估其在诊断和治疗提取物标准化中的潜在作用。已取得三个主要成果：1）鉴定患者血清中IgE和IgG识别的过敏原表位；2）通过亲和层析纯化相关过敏原成分；3）开发免疫测定法以定量用于诊断和治疗的市售提取物中的过敏原。
