Cohn J A, Melhus O, Page L J, Dittrich K L, Vigna S R
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Biochem Biophys Res Commun. 1991 Nov 27;181(1):36-43. doi: 10.1016/s0006-291x(05)81378-6.
Rabbit antisera were raised to six synthetic peptides corresponding to amino acid sequences contained in the protein product of the cystic fibrosis gene, CFTR. For two peptides, [Lys102]CFTR(102-116) and CFTR(1468-1480), antibody-peptide binding was of high affinity in that half-maximal binding occurred at peptide concentrations below 10 nM. Monospecific antibodies were prepared using these peptides, and these antibodies were used to stain human skin. Specific staining was detected in the cells lining the reabsorptive duct of the sweat gland. Within these lumenal cells, staining was most prominent at the apical domain but was also detected near the basolateral surface. This finding agrees well with predictions based on the effects of cystic fibrosis on sweat gland function, and suggests that these antibodies will be useful for studying CFTR in other human tissues.
用与囊性纤维化基因（CFTR）蛋白质产物中所含氨基酸序列相对应的六种合成肽制备了兔抗血清。对于两种肽，即[赖氨酸102]CFTR(102 - 116)和CFTR(1468 - 1480)，抗体 - 肽结合具有高亲和力，因为在肽浓度低于10 nM时发生半数最大结合。使用这些肽制备了单特异性抗体，并将这些抗体用于对人皮肤进行染色。在汗腺重吸收导管的内衬细胞中检测到特异性染色。在这些管腔细胞内，染色在顶端区域最为明显，但在基底外侧表面附近也能检测到。这一发现与基于囊性纤维化对汗腺功能影响的预测非常吻合，并表明这些抗体将有助于在其他人体组织中研究CFTR。
