Materniak M, Bicka L, Kuźmak J
Department of Biochemistry, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland.
Pol J Vet Sci. 2006;9(4):207-11.
The first isolation and partial characterization of bovine foamy virus (BFV), also known as bovine syncytial virus, in Poland is described. This virus was isolated by co-cultivation of peripheral blood leukocytes from infected cattle with permissive Cf2Th cells. The new isolate, called BFV100 was identified using several techniques: electron microscopy, western blotting, PCR and sequencing of a part of the gag and pol/env genes. Based on syncytia induction, antigenic determinants, primer binding sites and sequence analysis, it can be concluded that isolate BFV100 is bovine foamy virus and is related to the known American and German BFV isolates by sequence homology and antigenic relatedness.
本文描述了在波兰首次分离和部分鉴定牛泡沫病毒（BFV），也称为牛合胞体病毒。该病毒通过将感染牛的外周血白细胞与允许性Cf2Th细胞共同培养而分离得到。使用多种技术对新分离株BFV100进行了鉴定：电子显微镜、蛋白质印迹法、聚合酶链反应以及gag和pol/env基因部分序列的测序。基于合胞体诱导、抗原决定簇、引物结合位点和序列分析，可以得出结论，分离株BFV100是牛泡沫病毒，并且通过序列同源性和抗原相关性与已知的美国和德国BFV分离株相关。
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