日本牛群中泡沫病毒感染的调查及与牛白血病病毒感染的比较。
Survey of bovine foamy virus infection among cattle in Japan and comparison with bovine leukemia virus infection.
机构信息
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-0880, Japan.
Ibaraki Kenhoku Animal Hygiene Service Station, Nakakawauchi 966-1, Mito, Ibaraki 310-0002, Japan.
出版信息
J Vet Med Sci. 2020 May 20;82(5):615-618. doi: 10.1292/jvms.19-0592. Epub 2020 Mar 20.
The prevalence of bovine foamy virus (BFV) infections in cattle on farms in the Kanto region of Japan was determined using agar gel immunodiffusion (AGID) test and polymerase chain reaction (PCR). Six out of 20 farms contained BFV-positive cattle. Furthermore, 16.7% (91/545) of all cattle tested positive for BFV. This suggested that BFV-infected cattle are widely prevalent in Japan. Positive results for BFV infection were consistent between AGID and PCR tests. Additionally, we tested for bovine leukemia virus (BLV) infections at nine farms, primarily those containing BFV-infected cows. At each farm, the infection rate of BFV was lower than that of BLV. Further, cattle that were PCR-positive but antibody-negative, indicating immune tolerance to BFV, were not detected.
本研究采用琼脂凝胶免疫扩散（AGID）试验和聚合酶链反应（PCR）法，检测了日本关东地区农场牛群中泡沫病毒（BFV）的感染情况。20 个农场中有 6 个场的牛为 BFV 阳性。此外，545 头牛中有 16.7%（91/545）的牛 BFV 检测结果为阳性。这表明日本的 BFV 感染牛广泛存在。AGID 和 PCR 检测结果一致。此外，我们在 9 个主要含有 BFV 感染牛的农场检测了牛白血病病毒（BLV）感染情况。在每个农场，BFV 的感染率均低于 BLV。另外，未检测到对 BFV 具有免疫耐受的 PCR 阳性但抗体阴性的牛。
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