Hars Eszter S, Qi Haiyan, Ryazanov Alexey G, Jin Shengkan, Cai Li, Hu Chengcheng, Liu Leroy F
Department of Pharmacology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
Autophagy. 2007 Mar-Apr;3(2):93-5. doi: 10.4161/auto.3636. Epub 2007 Mar 30.
The role of autophagy in ageing regulation has been suggested based on studies in C. elegans, in which knockdown of the expression of bec-1 (ortholog of the yeast and mammalian autophagy genes ATG6/VPS30 and beclin 1, respectively) shortens lifespan of the daf-2(e1370) mutant C. elegans. However, Beclin1/ATG6 is also known to be involved in other cellular functions in addition to autophagy. In the current study, we knocked down two other autophagy genes, atg-7 and atg-12, in C. elegans using RNAi. We showed that RNAi shortened the lifespan of both wild type and daf-2 mutant C. elegans, providing strong support for a role of autophagy in ageing regulation.
基于对秀丽隐杆线虫的研究，自噬在衰老调控中的作用已被提出。在该研究中，敲低bec-1（分别为酵母和哺乳动物自噬基因ATG6/VPS30和Beclin 1的直系同源基因）的表达会缩短daf-2(e1370)突变型秀丽隐杆线虫的寿命。然而，众所周知，Beclin1/ATG6除了参与自噬外，还参与其他细胞功能。在本研究中，我们利用RNA干扰技术在秀丽隐杆线虫中敲低了另外两个自噬基因atg-7和atg-12。我们发现RNA干扰缩短了野生型和daf-2突变型秀丽隐杆线虫的寿命，为自噬在衰老调控中的作用提供了有力支持。
