Young I Ross, Rice Gregory E, Palliser Hannah K, Ayhan Mustafa, Dellios Nicole L, Hirst Jonathan J
Department of Physiology, Monash University, Victoria, Australia.
Proteomics. 2007 Jan;7(2):281-8. doi: 10.1002/pmic.200500705.
Preterm labour is a major problem in obstetrics. Timely intervention with available treatments is hampered by the lack of a reliable test of imminent preterm delivery. Current methods of diagnosis are based on the detection of breakdown products of foetal membranes or structural changes to the cervix when preterm labour is well established. The aim of this study was to screen the cervicovaginal fluid (CVF) proteome to identify labour-associated proteins that could be used as markers of imminent preterm delivery. Labour was induced in sheep at 135 days of gestation (term 147 days) by foetal infusion of dexamethasone (1 mg/24 h). CVF samples were collected before and 28 h after the start of infusion as well as at delivery (58.7 +/- 1.9 after the start of infusion, n = 5). One protein that was upregulated eight-fold, was bactenecin-1, a member of the cathelicidin family of antimicrobial proteins. This antimicrobial protein warrants further investigation as a marker of preterm labour, particularly during the period after the initiation of labour but before there is marked cervical connective tissue breakdown.
早产是产科的一个主要问题。由于缺乏可靠的即将早产的检测方法，现有治疗方法的及时干预受到阻碍。目前的诊断方法是基于胎膜破裂产物的检测或早产已确立时宫颈的结构变化。本研究的目的是筛查宫颈阴道液（CVF）蛋白质组，以鉴定可作为即将早产标志物的与分娩相关的蛋白质。在妊娠135天（足月147天）时，通过向胎儿输注地塞米松（1mg/24h）诱导绵羊分娩。在输注开始前、输注开始后28小时以及分娩时（输注开始后58.7±1.9小时，n = 5）收集CVF样本。一种上调了八倍的蛋白质是杀菌肽-1，它是抗菌蛋白cathelicidin家族的成员。这种抗菌蛋白作为早产的标志物值得进一步研究，特别是在分娩开始后但宫颈结缔组织明显分解之前的这段时间。
